Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis
Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis
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DOI：
10.1007/s00436-011-2759-8
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发表时间：
2012-06-01
影响因子：
2
通讯作者：
Yongvanit, Puangrat
中科院分区：
文献类型：
--
作者：
Juasook, Amornrat;Boonmars, Thidarut;Yongvanit, Puangrat
猫尾后睾吸虫是胆管癌（CCA）发展的危险因素之一，在包括泰国在内的东南亚地区流行。CCA由机械损伤、寄生虫分泌物和免疫病理学的组合引起的慢性炎症诱导。慢性感染O.灵猫病毒与几种影响肝胆癌和CCA发展的肝胆疾病有关。因此，减少了O.灵猫感染可能是降低胆管癌发生风险的选择之一。泼尼松龙是一种抑制炎症的甾体抗炎药，近年来其使用量不断上升。因此，我们研究了泼尼松龙对叙利亚仓鼠后睾吸虫病病理变化的影响，包括大体和组织病理学变化、蠕虫大小、每克卵数、每虫卵数和COX 2免疫组织化学染色。叙利亚仓鼠分为三组：未感染对照组、O. Viverrini-infected（OV）和O. Viverrini感染加泼尼松龙给药（OVP）。结果显示OVP组通过减少肝内胆管周围的炎性细胞而具有抗炎作用。然而，此外，所有观察时间的寄生虫大小均大于其他组，这也与每只蠕虫的卵数和每克粪便的卵数增加相关。这一结果表明，泼尼松龙是有用的抑制叙利亚仓鼠后睾吸虫病的炎症，而它也是有益的寄生虫，通过提高他们的生殖发育。为了阐明这一现象的机制，正在进行进一步的研究。
Opisthorchis viverrini is one of the risk factors for cholangiocarcinoma (CCA) development and is endemic in Southeast Asia including Thailand. CCA is induced by chronic inflammation from a combination of mechanical damage, parasite secretions, and immunopathology. Chronic infection with O. viverrini has been associated with several hepatobiliary diseases which affect the development of hepatobiliary cancer and CCA. Therefore, reducing the pathogenesis from O. viverrini infection may be one of the choices to reduce the risk of cholangiocarcinoma development. Prednisolone is one of the steroidal anti-inflammatory drugs that suppress inflammation, and its use has risen continuously in recent years. We therefore investigated the effect of prednisolone on pathological changes in Syrian hamster opisthorchiasis, in terms of gross and histopathological changes, worm size, eggs per gram, eggs per worm, and immunohistochemical staining for COX2. Syrian hamsters were divided into three groups: uninfected control; O. viverrini-infected (OV); and O. viverrini-infected plus prednisolone administration (OVP). The results showed an anti-inflammatory effect in the OVP group by a reduction of the inflammatory cells surrounding the intrahepatic bile ducts. However, in addition, parasite sizes for all times of observation were larger than for other groups, which was also correlated with increased eggs per worm and eggs per gram of feces. This result suggests that prednisolone is useful in suppressing inflammation in Syrian hamster opisthorchiasis, whereas it was also beneficial for parasites by enhancing their reproductive development. To clarify the mechanism of this phenomenon, further studies are under investigation.