Chromatoid bodies: Aggresome-like characteristics and degradation sites for organelles of spermiogenic cells
Chromatoid bodies: Aggresome-like characteristics and degradation sites for organelles of spermiogenic cells
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DOI：
10.1369/jhc.4a6520.2005
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发表时间：
2005-04-01
影响因子：
3.2
通讯作者：
Yokota, S
中科院分区：
文献类型：
--
作者：
Haraguchi, CM;Mabuchi, T;Yokota, S
我们研究了定位的几个标记的溶酶体和侵略者在染色体体（CB）的免疫电镜。我们在CB的核心发现了所谓的攻击性标记物，如Hsp70和泛素，以及CB周围的波形蛋白和蛋白酶体亚基。在CBs中还发现了泛素结合酶（E2）。在CBs周围的管泡状结构中，检测到溶酶体标记物，但没有检测到内质网滞留信号（KDEL）。此外，蛋白质位于每个亚细胞区室，包括细胞质，线粒体和细胞核，检测到的CB。在CB中也发现了编码在线粒体DNA上的细胞色素氧化酶I（COXI）的信号。标记密度的定量分析表明，所有检测的蛋白质都在一定程度上集中在CB中。这些结果表明CB具有一些侵略性特征，表明它们不是先前提出的合成位点，而是不必要的DNA、RNA和蛋白质被消化的降解位点。
We investigated the localization of several markers for lysosomes and aggresomes in the chromatoid bodies (CBs) by immunoelectron microscopy. We found so-called aggresomal markers such as Hsp70 and ubiquitin in the core of the CBs and vimentin and proteasome subunit around the CBs. Ubiquitin-conjugating enzyme (E2) was also found in the CBs. In tubulovesicular structures surrounding the CBs, lysosomal markers were detected but an endoplasmic reticulum retention signal (KDEL) was not. Moreover, proteins located in each subcellular compartment, including the cytosol, mitochondria, and nucleus, were detected in the CBs. Signals for cytochrome oxidase I (COXI) coded on mitochondrial DNA were also found in the CBs. Quantitative analysis of labeling density showed that all proteins examined were concentrated in the CBs to some extent. These results show that the CBs have some aggresomal features, suggesting that they are not a synthetic site as proposed previously but a degradation site where unnecessary DNA, RNA, and proteins are digested.