Solvent-free matrix dry-coating for MALDI Imaging of phospholipids
Solvent-free matrix dry-coating for MALDI Imaging of phospholipids
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DOI：
10.1016/j.jasms.2008.02.013
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Puolitaival, Satu M.;Burnum, Kristin E.;Caprioli, Richard M.
建立了一种快速、简便、无溶剂的基质沉积正离子模式磷脂分析方法。使用干涂层技术将细磨的2，5-二羟基苯甲酸沉积在矢状小鼠脑切片上，其中通过20 μ m不锈钢筛将固体基质颗粒直接过滤到组织上。直接从这些切片获得磷脂信号，从而获得高分辨率MS图像。将这些图像与用薄层色谱（TLC）试剂喷雾器喷涂的连续切片的图像进行比较。从干基质沉积法获得的信号与从喷涂切片获得的信号相当，产生相同的定位模式，具有更简单和更快的样品制备，几乎没有分析物离域。发现这种方法产生高度可重复的结果，消除了操作员差异引起的大部分差异，并使其成为目前使用的基质应用方法的有吸引力的替代方案。
A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-mu m stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods.