Treatment with ginseng total saponins improves the neurorestoration of rat after traumatic brain injury
Treatment with ginseng total saponins improves the neurorestoration of rat after traumatic brain injury
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人参总皂苷治疗改善大鼠脑外伤后神经恢复
DOI：
10.1016/j.jep.2014.07.009
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发表时间：
2014-09-11
影响因子：
5.4
通讯作者：
Peng, Bin
中科院分区：
文献类型：
--
作者：
Hu, Bao-Ying;Liu, Xian-Jin;Peng, Bin
民族药理学相关性：人参，人参的根。Meyer是一种传统草药，在亚洲被广泛用于治疗多种疾病，其作用是增强活力，增强身体对致病因素的抵抗力，产生体液和解渴，缓解身心不安和有益于智力，减轻体重和延长寿命。人参皂苷是人参中最重要的生物活性物质。大量研究表明，黄芪皂苷具有显著的神经保护和神经营养作用，能促进神经干/祖细胞（NSC）增殖，促进神经突起生长和神经网络形成。本研究的目的是探讨是否治疗与三七皂甙可以促进神经干细胞增殖海马结构创伤性脑损伤（TBI）后，有助于神经功能的恢复，包括学习和memory.Materials和方法：改良Feeney的方法是用来诱导TBI大鼠。人参总皂苷（GTS）在TBI后每天两次腹腔注射1周。结果：1）GTS（5-80 mg/kg）能明显改善创伤后神经功能的恢复，包括学习记忆功能的恢复，减少海马区神经元的丢失; 20、40、60和80 mg/kg的GTS效果优于5和10 mg/kg的GTS。2)GTS（20 mg/kg）可增加脑损伤后海马结构NGF、GDNF和NCAM的表达，抑制Nogo-A、Nogo-B、TN-C的表达，增加BrdU/nestin阳性神经干细胞数。结论：GTS可减轻脑损伤后继发性脑损伤，改善神经功能，其有效剂量范围为5-80 mg/kg。GTS可调节神经生长相关因子的表达，促进神经干/祖细胞的增殖，促进神经再生和组织修复，促进学习记忆等神经功能的恢复。人参总皂苷的这些作用可能为人参在传统医学中作为一种增智、延缓衰老、延年益寿的中草药提供了依据。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Ginseng, the root of Panax ginseng CA. Meyer, is a traditional medicinal herb that has been widely used in Asia for the treatment of many diseases through its effects of reinforcing vitality, strengthening the bodily resistance to pathogenic factors, engendering body liquids and allaying thirst, relieving uneasiness of the body and mind and benefiting intelligence, reducing body weight and prolonging life. Ginsenosides are the most important biologically active substances in ginseng. Many reports have suggested that ginsenosides could exert prominent neuroprotective and neurotrophic effects, promote neural stem/progenitor cell (NSC) proliferation and promote neurite outgrowth and neuronal network formation. The present study aimed to investigate whether treatment with ginsenosides could facilitate NSC proliferation in the hippocampal formation after traumatic brain injury (TBI) and contribute to the recovery of neurological functions including learning and memory.Materials and methods: The modified Feeney's method was used to induce a TBI in rats. Ginseng total saponins (GTS) were treated intraperitoneally twice a day for 1 week after the TBI. The neurological functions, morphology of the hippocampus, expression of nerve growth-related factors and number of NSCs in the hippocampal formation ipsilateral to the trauma were determined.Results: We determined 1) GTS (5-80 mg/kg) treatment after a TBI improved the recovery of neurological functions, including learning and memory, and reduced cell loss in the hippocampal area. The effects of GTS at 20,40,60, and 80 mg/kg were better than the effects of GTS at 5 and 10 mg/kg. 2) GTS treatment (20 mg/kg) after a TBI increased the expression of NGF, GDNF and NCAM, inhibited the expression of Nogo-A, Nogo-B, TN-C, and increased the number of BrdU/nestin positive NSCs in the hippocampal formation.Conclusions: GTS treatment in rats after a TBI alleviated the secondary brain injury and ameliorated the neurological functions with an effective dose limit of 5-80 mg/kg. GTS regulated the expression of nerve growth-related factors and improved the proliferation of neural stem/progenitor cells, which might facilitate neural regeneration and tissue repair, and might contribute to the recovery of neurological functions, including learning and memory. These effects of GTS might provide a foundation for the use of ginseng as a medicinal herb to enhance intelligence, reduce the aging process and prolong life in the traditional medicine. (C) 2014 Elsevier Ireland Ltd. All rights reserved.