Epinephrine yields translocation of lymphocytes to the lung
Epinephrine yields translocation of lymphocytes to the lung
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DOI：
10.1006/exmp.2000.2342
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发表时间：
2001-02-01
影响因子：
3.6
通讯作者：
Leary, C
中科院分区：
文献类型：
--
作者：
Kradin, R;Rodberg, G;Leary, C
为了检测肾上腺素能刺激对免疫淋巴细胞向肺运输的影响，给C57 BL/6小鼠皮下注射肾上腺素，观察肺、肺门淋巴结、脾、细胞荧光法淋巴细胞亚群分析表明肾上腺素增加了肺酶促反应中的“记忆”CD 4+淋巴细胞，但减少了它们在脾脏中的代表性。NK 1.1+淋巴细胞在肺中同时增加，在脾中减少。接下来，在肾上腺素注射前48小时，用Hoechst亲脂性红色染料PKH 26在体内标记脾或腘淋巴结中的淋巴细胞。肾上腺素注射后30分钟，肺组织中检测到PKH 26+淋巴细胞，而对照组中未检测到。心得安可抑制淋巴细胞的迁移。我们的结论是，肾上腺素促进免疫淋巴细胞从淋巴组织迅速转移到肺和血液。免疫淋巴细胞的定向运输可能是粘膜屏障表面对应激的适应性免疫应答的特征。(C)北京：科学出版社.
Previous studies have demonstrated that acute stress leads to the appearance of T-lymphocytes in skin and bone marrow In order to examine the effects of adrenergic stimulation on the traffic of immune lymphocytes to the lung, C57BL/6 mice were injected with epinephrine subcutaneously, and changes in lymphocyte representation in lung, hilar lymph node, spleen, and blood were examined at 30 min. Cytofluorimetric lymphocyte subset analysis showed that epinephrine increased "memory" CD4+ lymphocytes in enzymatic digests of lung but decreased their representation in spleen. NK1.1+ lymphocytes were concomitantly increased in lung and decreased in spleen. Next, lymphocytes in spleen or popliteal lymph nodes were labeled with the Hoechst lipophilic red dye PKH26 in vivo 48 h prior to epinephrine injection. PKH26 + lymphocytes were identified in lung tissue at 30 min postepinephrine but not in controls. Lymphocyte traffic was inhibited by propanolol. We conclude that epinephrine promotes rapid shifts in immune lymphocytes from lymphoid tissues into lung and blood. Directed traffic of immune lymphocytes may be a feature of the adaptive immune response to stress at mucosal barrier surfaces. (C) 2001 Academic Press.