Optical collection of extracellular vesicles in a culture medium enhanced by interactions with gold nanoparticles
Optical collection of extracellular vesicles in a culture medium enhanced by interactions with gold nanoparticles
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DOI：
10.1007/s44211-022-00207-2
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发表时间：
2022-11-05
影响因子：
1.6
通讯作者：
Kaneta，Takashi
中科院分区：
文献类型：
--
作者：
Tani，Yumeki;Ochiai，Kenta;Kaneta，Takashi
细胞外囊泡（EV）存在于生物流体如血液、尿液和脑脊液中，并且是有前途的癌症生物标志物。然而，分离和分析微量EV的尝试对于研究其功能和分泌机制的研究人员来说是一个挑战，这阻碍了诊断应用的选择。这项研究展示了一系列通过使用光学力由金纳米颗粒（AuNPs）增强的EV。收集系统包括一个倒置显微镜，配备了一个CCD摄像头，一个正方形毛细管连接聚四氟乙烯管，和一个Nd：YAG激光器，产生光的力量。激光束聚焦在毛细管壁上，其中含有EV的细胞培养基连续流动。毛细管壁和AuNP上的表面电荷的控制实现了EV在毛细管壁上的收集和保留。由于EV表面固有的负电荷，即使在激光照射停止后，带正电荷的毛细管壁仍保留EV。相反，带正电的金纳米粒子与电动汽车有很强的静电相互作用，并增强了作用在它们身上的光学力，这使得收集它们成为一个更有效的过程。图形摘要
Extracellular vesicles (EVs) exist in biological fluids such as blood, urine, and cerebrospinal fluid and are promising cancer biomarkers. Attempts to isolate and analyze trace EVs, however, have been a challenge for researchers studying their functions and secretion mechanisms, which has stymied the options for diagnostic application. This study demonstrated a collection of EVs that was enhanced by gold nanoparticles (AuNPs) via the use of optical force. The collection system consists of an inverted microscope equipped with a CCD camera, a square capillary connected with a PTFE tube, and an Nd:YAG laser that generates optical force. The laser beam was focused on a capillary wall in which a cell culture medium containing EVs flowed continuously. Control of the surface charges on both the capillary wall and the AuNPs achieved the collection and retention of EVs on the capillary wall. The positively charged capillary wall retained EVs even after the laser irradiation was halted due to the negative charges inherent on the surface of EVs. Conversely, positively charged AuNPs had a strong electrostatic interaction with EVs and enhanced the optical force acting on them, which made collecting them a much more efficient process.Graphical abstract