First isolation of Neospora caninum from the feces of a naturally infected dog
First isolation of Neospora caninum from the feces of a naturally infected dog
复制标题
DOI：
10.1645/0022-3395(2001)087
复制
发表时间：
2001-06-01
影响因子：
1.3
通讯作者：
Dubey, JP
中科院分区：
文献类型：
--
作者：
Basso, W;Venturini, L;Dubey, JP
犬新孢子虫是世界范围内牛流产的主要原因。牛通过摄入环境中的卵囊或经胎盘从母体传播给胎儿而感染犬新孢子虫。在实验中，犬可作为终末宿主，但犬在摄入组织包囊后排出的卵囊很少。直到现在，自然终末宿主一直未知。在本研究中，从一只犬的粪便中分离出犬新孢子虫。喂食该犬粪便的沙鼠（长爪沙鼠）在犬新孢子虫凝集试验中产生了针对犬新孢子虫的抗体，并且在其大脑中发现了组织包囊。在细胞培养以及接种感染沙鼠脑匀浆的γ - 干扰素基因敲除小鼠中分离出犬新孢子虫。从犬的粪便卵囊、喂食犬粪便的沙鼠大脑以及接种沙鼠大脑的细胞培养物中分离出的生物体所获得的DNA被确认为犬新孢子虫。通过生物测定和聚合酶链反应对犬新孢子虫卵囊的鉴定表明，犬是犬新孢子虫的自然终末宿主。
Neospora caninum is a major cause of abortion in cattle worldwide. Cattle become infected with N, caninum by ingesting oocysts from the environment or transplacentally from dam to fetus. Experimentally, dogs can act as definitive hosts, but dogs excrete few oocysts after ingesting tissue cysts. A natural definitive host was unknown until now. In the present study, N. caninum was isolated from the feces of a dog. Gerbils (Meriones unguiculatus) fed feces from the dog developed antibodies to N. caninum in the Neospora caninum agglutination test, and tissue cysts were found in their brains, Neospora caninum was isolated in cell culture and in gamma-interferon gene knockout mice inoculated with brain homogenates of infected gerbils. The DNA obtained from fecal oocysts of the dog, from the brains of gerbils fed dog feces, and from organisms isolated in cell cultures inoculated with gerbil brains was confirmed as N. caninum. The identification of N. caninum oocyst by bioassay and polymerase chain reaction demonstrates that the dog is a natural definitive host for N. caninum.