Use of c-myb antisense oligonucleotides to increase the sensitivity of human colon cancer cells to cisplatin.
Use of c-myb antisense oligonucleotides to increase the sensitivity of human colon cancer cells to cisplatin.
复制标题
使用c-myb反义寡核苷酸提高人结肠癌细胞对顺铂的敏感性。
DOI：
10.3892/or.8.4.807
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Kaku
中科院分区：
文献类型：
--
作者：
T. Funato;J. Satou;K. Kozawa;S. Fujimaki;T. Miura;M. Kaku
人结肠癌SW 480 DDP和SW 620 DDP顺铂耐药细胞c-myb基因表达较亲本细胞SW 480和SW 620强。然而，顺铂耐药细胞系中的细胞生长速率与亲本细胞的生长速率相似。c-myb反义寡核苷酸抑制c-myb表达，并诱导增加顺铂在SW 480 DDP和SW 620 DDP细胞的敏感性，但这并没有发生与控制正义寡核苷酸。相反，亲本细胞系不受c-myb反义寡核苷酸的影响。这些结果表明，c-myb基因在人类结肠癌顺铂耐药的相关因素之一，并支持需要开发抗癌治疗的基础上癌基因靶向反义寡核苷酸技术。
Human colon cancer SW480DDP and SW620DDP cells resistant to cisplatin exhibited stronger c-myb gene expression than the parent SW480 and SW620 cells. However, cell growth rates in the cisplatin-resistant cell lines remained similar to those of the parent cells. Antisense oligonucleotides to c-myb inhibited c-myb expression and induced increased sensitivity to cisplatin in SW480DDP and SW620DDP cells, but this did not occur with the control sense oligonucleotides. In contrast, the parent cell lines were not affected by antisense oligonucleotides to c-myb. These results indicate that the c-myb gene in human colon cancer is one of the factors related to cisplatin resistance, and support the need to develop anti-cancer therapeutics based on oncogene-targeted antisense oligonucleotide technology.