Evolution of a plant growth-regulatory protein interaction specificity.
Evolution of a plant growth-regulatory protein interaction specificity.
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DOI：
10.1038/s41477-023-01556-0
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发表时间：
2023-12
期刊：
影响因子：
18
通讯作者：
Harberd, Nicholas P.
中科院分区：
文献类型：
--
作者：
Ji, Zhe;Belfield, Eric J.;Zhang, Siyu;Bouvier, Jacques;Li, Shan;Schnell, Jason;Fu, Xiangdong;Harberd, Nicholas P.
特定的蛋白质-蛋白质相互作用(PPI)使生物调节成为可能。然而，人们对PPI特异性的演变知之甚少。在这里，我们跟踪了陆地-植物生长调节della-SLY1/GID2 PPI的演变，揭示了SLY1/GID2对特定della形式的亲和力的特异性逐渐增加。早期分化的SLY1表现出相对广泛的della亲和力，而后期分化的SLY1往往对由促进生长的植物激素赤霉素(GA)产生的特定della A‘形式具有越来越严格的亲和力。我们新的突变策略揭示了氨基酸替代有助于拟南芥SLY1A的特异性进化，也表明允许回复到更广泛亲和力的路线随着进化时间的推移变得越来越受限制。我们认为，亲和力的逐渐缩小可能是PPI专一性的一个重要进化驱动因素，SLY1/GID2-DELLA专一性的增加使GA-DELLA生长调节机制赋予植物生理环境适应的灵活性增强。纪万昌及其同事证明，特定的被子植物生长控制的della-SLY1/GID2蛋白相互作用是从更广泛的祖先亲缘关系演变而来的，这表明亲和力缩小是相互作用专一性的一般进化驱动因素。
Specific protein–protein interactions (PPIs) enable biological regulation. However, the evolution of PPI specificity is little understood. Here we trace the evolution of the land-plant growth-regulatory DELLA–SLY1/GID2 PPI, revealing progressive increase in specificity of affinity of SLY1/GID2 for a particular DELLA form. While early-diverging SLY1s display relatively broad-range DELLA affinity, later-diverging SLY1s tend towards increasingly stringent affinity for a specific DELLA A’ form generated by the growth-promoting phytohormone gibberellin (GA). Our novel mutational strategy reveals amino acid substitutions contributing to the evolution of Arabidopsis thaliana SLY1 A’ specificity, also showing that routes permitting reversion to broader affinity became increasingly constrained over evolutionary time. We suggest that progressive affinity narrowing may be an important evolutionary driver of PPI specificity and that increase in SLY1/GID2-DELLA specificity enabled the enhanced flexibility of plant physiological environmental adaptation conferred by the GA-DELLA growth-regulatory mechanism. Ji and colleagues show that the specific angiosperm growth-governing DELLA–SLY1/GID2 protein interaction evolved from a broader ancestral affinity, suggesting affinity narrowing to be a general evolutionary driver of interaction specificity.
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影响因子：
11.6
作者：
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通讯作者：
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影响因子：
18
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影响因子：
64.8
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
10.7
作者：
Hernandez-Garcia, Jorge;Briones-Moreno, Asier;Blazquez, Miguel A.
通讯作者：
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