Proteomic analysis of amniotic membrane prepared for human transplantation: Characterization of proteins and clinical implications
Proteomic analysis of amniotic membrane prepared for human transplantation: Characterization of proteins and clinical implications
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DOI：
10.1021/pr050425q
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发表时间：
2006-09-01
影响因子：
4.4
通讯作者：
Dua, Harminder S.
中科院分区：
文献类型：
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作者：
Hopkinson, Andrew;McIntosh, Richard S.;Dua, Harminder S.
羊膜通常用于几种外科手术。尽管有冷冻保存期，但据报道，它保留了伤口愈合、抗血管生成、再生和抗瘢痕形成的特性;然而，对活性蛋白含量知之甚少。对准备移植的羊膜（transplant-ready amniotic membrane，TRAM）进行2-DE分析。研究了保存和处理对羊膜蛋白质组的影响，并利用质谱和免疫印迹技术对羊膜TRAM中的主要蛋白质进行了表征。这鉴定了一系列蛋白质，包括血小板反应蛋白、mimecan、BIG-H3和整合素α 6。保存损害细胞活力，导致可溶性细胞蛋白的选择性洗脱，留下细胞外基质相关蛋白和细胞结构蛋白。在AM中证实了与眼表结构共同的许多关键结构蛋白，其参与稳态和伤口愈合。处理程序改变了准备用于移植的羊膜的蛋白质组成。如果没有标准化，将存在膜间变异，这可能损害移植准备好的羊膜的期望治疗效果。
Amniotic membrane is commonly exploited in several surgical procedures. Despite a freeze preservation period, it is reported to retain wound healing, anti-angiogenic, antiinflammatory and anti-scarring properties; however, little is known about the active protein content. 2-DE analysis of transplant-ready amniotic membrane ( TRAM) was performed. The effects of preservation and processing on amnion proteome were investigated, and the major proteins in the TRAM characterized using mass spectrometry and immunoblotting. This identified a spectrum of proteins including thrombospondin, mimecan, BIG-H3, and integrin alpha 6. Preservation compromises cellular viability resulting in selective elution of soluble cellular proteins, leaving behind extracellular matrix-associated and cell structural proteins. A number of key architectural proteins common to the architecture of the ocular surface were demonstrated in AM, which are involved in homeostasis and wound healing. Handling procedures alter the protein composition of amniotic membrane prepared for transplantation. Without standardization, there will be inter-membrane variation, which may compromise the desired therapeutic effect of transplant ready amniotic membrane.