Bilateral alteration in stepping pattern after unilateral motor cortex injury: A new test strategy for analysis of skilled limb movements in neurological mouse models
Bilateral alteration in stepping pattern after unilateral motor cortex injury: A new test strategy for analysis of skilled limb movements in neurological mouse models
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DOI：
10.1016/j.jneumeth.2005.10.011
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发表时间：
2006-05-15
影响因子：
3
通讯作者：
Metz, Gerlinde A.
中科院分区：
文献类型：
--
作者：
Farr, Tracy D.;Liu, Lily;Metz, Gerlinde A.
小鼠正变得越来越受欢迎，以模拟神经系统疾病和运动系统功能障碍。对于离散的慢性运动障碍的评估，熟练的肢体运动代表了标准小鼠测试电池的有价值的扩展。本研究介绍了一种有效的和敏感的测试策略，全面评估熟练的前肢和后肢步进小鼠。成年C57 BL/6小鼠在两个行走任务中进行训练和视频记录，广泛使用的旋转杆测试和新的梯子横档任务。然后，动物在运动皮质前肢和后肢区域接受单侧缺血性损伤，并在损伤后第12天和第26天进行视频记录。结合终点测量和定性评估对前肢和后肢步进运动进行评级。结果表明，虽然动物保持负重步态，但在两个术后时间段，姿势和步进运动均异常。旋转杆分析显示，步进赤字在两个前肢，导致通过补偿运动策略。梯子横档任务显示，同侧和对侧的前，后肢的步进错误。研究结果表明，这种测试策略提供了全面的评估运动障碍的小鼠和定性运动分析是一个有价值的工具，阐述微妙的运动障碍。(c)2005 Elsevier B. V.保留所有权利。
Mice are becoming increasingly popular to model neurological disease and motor system dysfunction. For evaluation of discrete, chronic motor impairments, skilled limb movements represent a valuable extension of standard mouse test batteries. This study introduces an efficient and sensitive test strategy for comprehensive assessment of skilled fore- and hind-limb stepping in mice. Adult C57BL/6 mice were trained and video-recorded in two walking tasks, the widely used rotorod test and a new ladder rung task. The animals then received a unilateral ischemic lesion in the motor cortex forelimb and hind limb area and were video-recorded on days 12 and 26 post-lesion. Forelimb and hind limb stepping movements were rated using a combination of endpoint measures and qualitative assessment. The results showed that while animals maintained a weight-supported gait, posture and stepping movements were abnormal at both post-operative intervals. The rotorod analysis revealed stepping deficits in both forelimbs that led to adoption of compensatory movement strategies. The ladder rung task revealed stepping errors in ipsi- and contralateral fore- and hind-limbs. The findings demonstrate that this test strategy provides comprehensive assessment of motor impairments in mice and that qualitative movement analysis is a valuable tool for elaborating subtle motor disturbances. (c) 2005 Elsevier B.V. All rights reserved.