Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system
Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system
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DOI：
10.1128/jvi.79.3.1906-1910.2005
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发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Lu, S
中科院分区：
文献类型：
--
作者：
Wang, SX;Chou, THW;Lu, S
严重急性呼吸综合征相关冠状病毒（SARS-CoV）的刺突蛋白（Spike，S）在病毒的致病机制中起重要作用，并可能在开发有效的疫苗对抗这种致命的传染病中发挥重要作用。本研究合成了经密码子优化的SARS冠状病毒S基因，构建了表达SARS冠状病毒S蛋白的DNA疫苗质粒。高滴度的S-特异性免疫球蛋白G抗体的反应，引起了在兔与DNA免疫的S蛋白的各个部分。在S蛋白上鉴定出两个中和结构域，一个在N末端（Ser 12-Thr 535），另一个在C末端附近（Arg 797-Ile 1192）。
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).