SS40 - THE ZINC ENDOPEPTIDASE SECRETED BY INFECTIVE LARVAE OF STRONGLOIDES STERCORALIS
SS40 - THE ZINC ENDOPEPTIDASE SECRETED BY INFECTIVE LARVAE OF STRONGLOIDES STERCORALIS
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DOI：
10.1006/expr.1995.1001
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发表时间：
1995-02-01
影响因子：
2.1
通讯作者：
NEVA, FA
中科院分区：
文献类型：
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作者：
BRINDLEY, PJ;GAM, AA;NEVA, FA
感染性幼虫的致病性线虫的人类，粪类圆线虫，释放一种有效的锌内肽酶活性，其具有广泛的底物特异性的成分的细胞外真皮基质，包括弹性蛋白。锌内肽酶的特异性抑制剂可防止链球菌对哺乳动物皮肤的渗透。粪蝇幼虫我们现在报告的分子大小和等电点的S。粪菌锌内肽酶的分子量为40 kDa，pI为5。的活动是不受影响的孵育与P-巯基乙醇在22摄氏度，但被灭活，在100摄氏度孵育2分钟。该酶，我们称之为Ss 40，是免疫原性和刺激体液IgG抗体在感染的人;活动是免疫沉淀从ES合并感染血清。此外，HPLC富集的Ss 40制剂刺激组胺从S.粪感染的人，这表明Ss 40是人类过敏原。(C)出版社：Academic Press
Infective larvae of a pathogenic nematode of humans, Strongyloides stercoralis, release a potent zinc endopeptidase activity which has a broad substrate specificity for constituents of extracellular dermal matrix, including elastin. Specific inhibitors of zinc endopeptidases prevent the penetration of mammalian skin by S. stercoralis larvae. We now report the molecular size and isoelectric point of the S. stercoralis zinc endopeptidase at 40 kDa, pI 5 by zymogram analysis. The activity was not influenced by incubation with P-mercaptoethanol at 22 degrees C, but was inactivated by incubation at 100 degrees C for 2 min. The enzyme, which we term Ss40, is immunogenic and stimulates humoral IgG antibodies during infection of humans; the activity was immunoprecipitable from ES with pooled infection sera. In addition, a HPLC-enricbed Ss40 preparation stimulated the release of histamine from peripheral blood leukocytes of S. stercoralis-infected persons, suggesting that Ss40 is allergenic in humans. (C) 1995 Academic Press, Inc.