Optogenetic Control of Gene Expression in Drosophila.
Optogenetic Control of Gene Expression in Drosophila.
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DOI：
10.1371/journal.pone.0138181
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kravitz EA
中科院分区：
文献类型：
--
作者：
Chan YB;Alekseyenko OV;Kravitz EA
为了研究复杂生物系统的分子机制，重要的是能够在期望的靶位点以高精度人工操纵基因表达。基于隐花色素2和隐花色素相互作用的bHLH蛋白的光依赖性结合，我们开发了一种用于果蝇的分裂莱克萨转录激活系统，该系统允许使用蓝光或双光子激发来调节体内基因表达。我们表明，该系统提供了高的时空分辨率诱导基因表达的组织在不同的发展阶段。结合双光子激发，可以在胚胎的精确位点操纵基因表达，这可能为研究发育过程提供了一个重要的工具。
To study the molecular mechanism of complex biological systems, it is important to be able to artificially manipulate gene expression in desired target sites with high precision. Based on the light dependent binding of cryptochrome 2 and a cryptochrome interacting bHLH protein, we developed a split lexA transcriptional activation system for use in Drosophila that allows regulation of gene expression in vivo using blue light or two-photon excitation. We show that this system offers high spatiotemporal resolution by inducing gene expression in tissues at various developmental stages. In combination with two-photon excitation, gene expression can be manipulated at precise sites in embryos, potentially offering an important tool with which to examine developmental processes.