Differential gene expression associated with fungal trophic shifts along the senescence gradient of the moss Dicranum scoparium
Differential gene expression associated with fungal trophic shifts along the senescence gradient of the moss Dicranum scoparium
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DOI：
10.1111/1462-2920.14605
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发表时间：
2019-07-01
影响因子：
5.1
通讯作者：
Lutzoni, Francois
中科院分区：
文献类型：
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作者：
Chen, Ko-Hsuan;Liao, Hui-Ling;Lutzoni, Francois
苔藓植物含有微生物组，包括各种真菌群落。多年生藓类与这些真菌的合作伙伴沿着其衰老梯度相互作用的分子机制是未知的，但这是一个理想的系统，研究与营养状态转换相关的基因表达的变化。我们研究了差异表达的基因的真菌群落和它们的宿主Dicranum scoparium在其自然发生的衰老梯度使用metatranscriptomic方法。真菌营养相关的（碳，氮，磷和硫）转运蛋白和碳水化合物活性酶（CAZy）基因的活性更高的检测对底部，部分分解，层的苔藓。真菌营养转运蛋白表达水平最显着的变化来自无机氮相关转运蛋白，而有机氮的分解被检测为宿主D最丰富的基因本体术语。scoparium，对于那些在部分分解层中具有较高表达的转录本。细菌rRNA转录本的丰富表明，更多的蓝细菌的生活成员与光合层D。scoparium，而成员的根瘤菌检测整个配子体。特定的真菌-植物通信，包括防御反应，植物基因的差异表达，表明不同的遗传途径参与植物微生物串扰在光合组织相比，部分分解的组织。
Bryophytes harbour microbiomes, including diverse communities of fungi. The molecular mechanisms by which perennial mosses interact with these fungal partners along their senescence gradients are unknown, yet this is an ideal system to study variation in gene expression associated with trophic state transitions. We investigated differentially expressed genes of fungal communities and their host Dicranum scoparium across its naturally occurring senescence gradient using a metatranscriptomic approach. Higher activity of fungal nutrient-related (carbon, nitrogen, phosphorus and sulfur) transporters and Carbohydrate-Active enZyme (CAZy) genes was detected toward the bottom, partially decomposed, layer of the moss. The most prominent variation in the expression levels of fungal nutrient transporters was from inorganic nitrogen-related transporters, whereas the breakdown of organonitrogens was detected as the most enriched gene ontology term for the host D. scoparium, for those transcripts having higher expression in the partially decomposed layer. The abundance of bacterial rRNA transcripts suggested that more living members of Cyanobacteria are associated with the photosynthetic layer of D. scoparium, while members of Rhizobiales are detected throughout the gametophytes. Plant genes for specific fungal-plant communication, including defense responses, were differentially expressed, suggesting that different genetic pathways are involved in plant-microbe crosstalk in photosynthetic tissues compared to partially decomposed tissues.