Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model
Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model
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DOI：
10.1117/1.1648646
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发表时间：
2004-03-01
影响因子：
3.5
通讯作者：
Tromberg, BJ
中科院分区：
文献类型：
--
作者：
Yeh, AT;Kao, BS;Tromberg, BJ
使用光学相干断层扫描（OCT）、多光子显微镜（MPM）和组织病理学监测器官型、皮肤等效组织模型中的激光热损伤和随后的伤口愈合。体外皮肤等效筏组织模型由含有I型胶原和成纤维细胞的真皮和分化的角质形成细胞的表皮组成。无创光学成像技术用于基质破坏和重建的时间依赖性连续测量，并与组织病理学进行比较。 OCT 图像中低信号强度清楚地描绘出激光热损伤区域。使用二次谐波生成的高分辨率 MPM 成像揭示了胶原微结构组织的变化以及随后的基质重建。 MPM 使用双光子激发荧光监测成纤维细胞对损伤的迁移。这项研究说明了内在信号光学成像中线性和非线性光组织相互作用的互补特征及其在生物组织中伤口愈合过程的无创连续监测中的用途。 (C) 2004 年光电仪器工程师协会。
Laser thermal injury and subsequent wound healing in organotypic, skin-equivalent tissue models were monitored using optical coherence tomography (OCT), multiphoton microscopy (MPM), and histopathology. The in vitro skin-equivalent raft tissue model was composed of dermis with type I Collagen and fibroblast cells and epidermis of differentiated keratinocytes. Noninvasive optical imaging techniques were used for time-dependent, serial measurements of matrix destruction and reconstruction and compared with histopathology. The region of laser thermal injury was clearly delineated in OCT images by low signal intensity. High resolution MPM imaging using second-harmonic generation revealed alterations in collagen microstructure organization with subsequent matrix reconstruction. Fibroblast cell migration in response to injury was monitored by MPM using two-photon excited fluorescence. This study illustrates the complementary features of linear and nonlinear light-tissue interaction in intrinsic signal optical imaging and their use for noninvasive, serial monitoring of wound healing processes in biological tissues. (C) 2004 Society of Photo-Optical Instrumentation Engineers.