Escherichia coli ribosomal 5S RNA-protein L25 nucleoprotein complex: effects of RNA binding on the protein structure and the nature of the interaction.
Escherichia coli ribosomal 5S RNA-protein L25 nucleoprotein complex: effects of RNA binding on the protein structure and the nature of the interaction.
复制标题
大肠杆菌核糖体 5S RNA-蛋白 L25 核蛋白复合物：RNA 结合对蛋白质结构和相互作用性质的影响。
DOI：
10.1021/bi00303a017
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Moore,PB
中科院分区：
文献类型：
--
作者：
Kime,MJ;Moore,PB
Matthew J. Kime和Peter B.摩尔 * 摘要：用高场质子核磁共振研究了大肠杆菌5S RNA的三种变体与核糖体蛋白L25的复合物。本文提出了一种光谱学方法来帮助区分核蛋白复合物中质子共振的大分子来源。L25结合对三种RNA的影响很小，L25的存在对RNA的构象没有强烈的影响。L25与5S RNA的相互作用产生了适度但独特的改变，
Matthew J. Kime and Peter B. Moore* abstract: The complexes of three variants of Escherichia coli 5S RNA with ribosomal protein L25 have been studied by high-field proton nuclear magnetic resonance. A spec-troscopic method is demonstrated to help distinguish the macromolecular sources of proton resonances in nucleoprotein complexes. The effects of L25 binding on the three RNAs tested were small; thepresence of the L25 did not strongly influence the conformation of the RNA. The interaction of L25 with 5S RNA produced modest, but distinctive, alterations