Presence of aquaporin-4 and muscarinic receptors in astrocytes and ependymal cells in rat brain: a clue to a common function?
Presence of aquaporin-4 and muscarinic receptors in astrocytes and ependymal cells in rat brain: a clue to a common function?
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DOI：
10.1016/s0304-3940(00)01364-1
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发表时间：
2000-10-06
影响因子：
2.5
通讯作者：
Lasbennes, F
中科院分区：
文献类型：
--
作者：
Badaut, J;Verbavatz, JM;Lasbennes, F
使用联合免疫荧光和激光共聚焦显微镜，我们研究了大鼠大脑皮层、胼胝体和室管膜细胞中胆碱能毒蕈碱受体 (mAChR) 和水通道蛋白 4 水通道 (AQP4) 的常见细胞定位。在皮质中，AQP4 染色仅限于星形胶质细胞的血管周围端足。更广泛地分布在胼胝体的星形胶质细胞上。在星形胶质细胞上，mAChR 经常存在于与 AQP4 发生免疫反应的区域。第三脑室边界的室管膜细胞也被两种抗体染色。 mAChRs 与 AQP4 对两种不同细胞类型的双重染色可能表明存在进一步的相互作用，这对于大脑中水和电解质运动的调节可能很重要。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
Using combined double immunofluorescence and laser confocal microscopy, we studied the common cellular localization of cholinergic muscarinic receptors (mAChRs) and aquaporin-4 water channels (AQP4) in the cortex, the corpus callosum and in ependymal cells of the rat brain. In the cortex, AQP4 staining was restricted to the perivascular end-feet of astrocytes. It was more widely distributed on the astrocytes of the corpus callosum. On astrocytes, mAChRs were often present in regions immunoreactive to AQP4. Ependymal cells bordering the third ventricle were also stained by both antibodies. The double staining of mAChRs with AQP4 on two different cell-types might indicate that further interactions exist which may be important in the regulation of water and electrolyte movements in the brain. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.