BacStalk: A comprehensive and interactive image analysis software tool for bacterial cell biology
BacStalk: A comprehensive and interactive image analysis software tool for bacterial cell biology
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DOI：
10.1111/mmi.14501
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发表时间：
2020-04-14
影响因子：
3.6
通讯作者：
Drescher, Knut
中科院分区：
文献类型：
--
作者：
Hartmann, Raimo;van Teeseling, Muriel C. F.;Drescher, Knut
原核细胞显示出惊人的亚细胞组织。对不同物种的潜在机制的研究极大地增强了我们对细菌对不同环境生态位的形态和生理适应的理解。图像分析软件工具BacStalk旨在从具有柄、鞭毛或其他附属物的形态复杂的细菌的图像中提取全面的定量信息。所得到的数据可以在交互式人口统计图、记波图、细胞谱系图和散点图中可视化，以实现快速和全面的数据分析和表示。值得注意的是，BacStalk可以生成在二维细胞轮廓内显示荧光信号的人口统计图和记波图，以准确地表示它们的亚细胞位置。除了具有可见附属物的生物外，BacStalk还适用于具有常见或不常见细胞形状的已建立的无柄模式生物。因此，BacStalk有助于原核细胞生物学和生理学的进步，因为它拓宽了易于访问的模型生物的范围，并实现了高度直观和交互式的数据分析和可视化。
Prokaryotic cells display a striking subcellular organization. Studies of the underlying mechanisms in different species have greatly enhanced our understanding of the morphological and physiological adaptation of bacteria to different environmental niches. The image analysis software tool BacStalk is designed to extract comprehensive quantitative information from the images of morphologically complex bacteria with stalks, flagella, or other appendages. The resulting data can be visualized in interactive demographs, kymographs, cell lineage plots, and scatter plots to enable fast and thorough data analysis and representation. Notably, BacStalk can generate demographs and kymographs that display fluorescence signals within the two-dimensional cellular outlines, to accurately represent their subcellular location. Beyond organisms with visible appendages, BacStalk is also suitable for established, non-stalked model organisms with common or uncommon cell shapes. BacStalk, therefore, contributes to the advancement of prokaryotic cell biology and physiology, as it widens the spectrum of easily accessible model organisms and enables highly intuitive and interactive data analysis and visualization.