Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.
Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.
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DOI：
10.3969/j.issn.2095-3941.2012.02.004
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发表时间：
2012-06
影响因子：
5.5
通讯作者：
Sun BC
中科院分区：
文献类型：
--
作者：
Zheng HY;Zhang XY;Wang XF;Sun BC
目的研究LC 3B-II和活性caspase-3在结直肠癌中的表达，探讨自噬在结直肠癌中的作用及其与细胞凋亡的关系。采用免疫组化法检测53例结直肠癌组织和20例正常结肠组织中LC 3B的表达。采用Western blot方法检测23例结直肠癌组织和10例正常结肠组织中LC 3B-II和活性caspase-3的蛋白水平。LC 3B在癌细胞和正常上皮细胞中均有表达。LC 3B表达与肿瘤分化程度（P=0.002）、肿瘤边缘生长方式（P = 0.028）、pN（P= 0.002）、pStage（P = 0.032）、血管和神经丛浸润（P=0.002）等临床病理因素有关。癌组织中LC 3B-II蛋白表达水平显著高于正常组织（P=0.038），而活性型半胱天冬酶-3蛋白表达水平显著低于正常组织（P=0.041）。LC 3B-II的表达水平与procaspase-3的活性形式之间存在统计学上显著的正相关性（r=0.537，P=0.008）。自噬在人类结直肠癌中具有促生存作用。自噬增强结直肠癌细胞的侵袭性和它们适应凋亡刺激的能力。
To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer. LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues. LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008). Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.
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DOI：
10.1016/j.bbrc.2009.03.051
发表时间：
2009-05-01
影响因子：
3.1
作者：
Wu, Ya Chun;Wu, William Ka Kei;Cho, Chi Hin
通讯作者：
Cho, Chi Hin
影响因子：
5.7
作者：
Fujii, Satoshi;Mitsunaga, Shuichi;Ochiai, Atsushi
通讯作者：
Ochiai, Atsushi
影响因子：
4
作者：
Eskelinen, Eeva-Liisa
通讯作者：
Eskelinen, Eeva-Liisa
自噬相关蛋白 Beclin-1 和 LC3 预测西妥昔单抗对晚期结直肠癌的疗效
影响因子：
4.3
作者：
Guo, Gui-Fang;Jiang, Wen-Qi;Xia, Liang-Ping
通讯作者：
Xia, Liang-Ping
影响因子：
5.3
作者：
Pan, Ji-An;Ullman, Erica;Zong, Wei-Xing
通讯作者：
Zong, Wei-Xing