Sam68 expression and cytoplasmic localization is correlated with lymph node metastasis as well as prognosis in patients with early-stage cervical cancer
Sam68 expression and cytoplasmic localization is correlated with lymph node metastasis as well as prognosis in patients with early-stage cervical cancer
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Sam68表达及胞质定位与早期宫颈癌患者淋巴结转移及预后相关
DOI：
10.1093/annonc/mdr290
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发表时间：
2012-03-01
影响因子：
50.5
通讯作者：
Song, L. -B.
中科院分区：
文献类型：
--
作者：
Li, Z.;Yu, C. -P.;Song, L. -B.
背景资料：本研究旨在探讨Sam 68在宫颈癌淋巴结转移中的作用及其分子机制，材料与方法：采用定量聚合酶链反应（PCR）、免疫印迹（Western blotting）和免疫组化染色检测Sam 68在宫颈癌淋巴结转移中的表达。结果：宫颈癌组织中Sam 68的表达在mRNA水平和蛋白水平均显著高于正常宫颈组织，并与正常宫颈组织中Sam 68的表达呈负相关。Sam 68高表达及其胞浆定位与盆腔淋巴结转移等危险因素显著相关（P < 0.001），可作为预测早期宫颈癌患者总生存期和无病生存期缩短的独立预后因素。此外，下调宫颈癌细胞中的Sam 68显著抑制细胞运动和侵袭。结论：Sam 68可通过调节上皮-间质转化诱导宫颈癌淋巴结转移，其表达谱可作为宫颈癌患者盆腔淋巴结转移的预测指标。
Background: This study was aimed at investigating the role and molecular mechanism of Sam68 in cervical cancer lymph node metastasis.Materials and methods: Sam68 expression profile was detected by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Short hairpin RNA interfering approach was employed to suppress endogenous Sam68 expression in cervical cancer cells to determine its role in metastasis and the possible mechanism.Results: Sam68 expression in cervical cancer was significantly up-regulated at both messenger RNA and protein levels compared with that in normal cervical tissues. The high expression level of Sam68 and its cytoplasmic localization were significantly associated with risk factors including pelvic lymph node metastasis (P < 0.001), and served as independent prognostic factors for predicting shortening of the overall survival time and disease-free survival time in patients with early-stage cervical cancer. Moreover, down-regulation of Sam68 in cervical cancer cells remarkably inhibited cellular motility and invasion. In addition, down-regulation of Sam68 reversed epithelial-mesenchymal transition through inhibiting the Akt/ GSK-3 beta/Snail pathway.Conclusion: This study demonstrated that Sam68 could induce cervical cancer lymph node metastasis through regulating epithelial-mesenchymal transition, and Sam68 expression profile possessed the potential to serve as predictors of pelvic lymph node metastasis in cervical cancer patients.