Photodynamic inactivation of microorganisms present on complete dentures. A clinical investigation
Photodynamic inactivation of microorganisms present on complete dentures. A clinical investigation
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全口义齿上微生物的光动力灭活。
DOI：
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发表时间：
2011
影响因子：
2.1
通讯作者：
C. Vergani
中科院分区：
文献类型：
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作者：
D. Ribeiro;A. C. Pavarina;L. Dovigo;Ewerton Garcia Oliveira Mima;A. Machado;V. Bagnato;C. Vergani
本研究的目的是评价光动力疗法(PDT)对全口义齿的消毒效果。从60名义齿使用者的义齿上采集生物膜样本，随机分为4组(n = 15)：上颌总义齿喷洒50、100 mg/L的PHOTOGEM®混悬液(P50S、P100S组)和上颌总义齿喷洒50、100 mg/L的PHOTOGEM®凝胶(P50G、P100G组)。将含有光敏剂的义齿置于黑暗中30min(预照射时间)，然后用蓝光以37.5J/cm2的速度照射(26min)。用无菌棉签分别于PDT前(左侧表面)和PDT后(右侧表面)采集义齿样本。将所有微生物材料稀释后，在念珠菌、变形葡萄球菌、链球菌和非选择性培养基上进行电镀。培养48h/37℃后，计数集落形成单位数(CFU/ml)。采用生化方法对PDT前后选择性培养基上的微生物进行鉴定。数据提交给麦克内马尔检验和克鲁斯卡尔-瓦利斯检验(α = 0.05)。P100G组、P50G组、P100S组和P50S组分别有60、53、47和40%的义齿在PDT后无生长。当观察到微生物生长的证据时，PDT方案消除了义齿上90%以上的微生物。这项临床研究表明，PDT对义齿消毒是有效的。
The aim of this study was to evaluate the effectiveness of photodynamic therapy (PDT) for the disinfection of complete dentures. Biofilm samples were collected from dentures of 60 denture users who were randomly divided into four experimental groups (n = 15 each): subjects whose maxillary dentures were sprayed with 50 and 100 mg/l of Photogem® suspension (groups P50S and P100S) and patients whose maxillary dentures were treated with 50 and 100 mg/l of Photogem® gel (groups P50G and P100G). Dentures with photosensitizers were left in the dark for 30 min (pre-irradiation time) and then irradiated with blue LED light at 37.5 J/cm2 (26 min). Denture samples were taken with sterile cotton swab before (left side surfaces) and after (right side surfaces) PDT. All microbial material was diluted and plated on selective media for Candida spp., Staphylococcus mutans spp., streptococci and a non-selective media. After incubation (48 h/37°C), the number of colony-forming units (cfu/ml) was counted. Microorganisms grown on selective media were identified using biochemical methods before and after PDT. The data were submitted to McNemar and Kruskal–Wallis tests (α = 0.05). No growth after PDT was observed in 60, 53, 47, and 40% of dentures from P100G, P50G, P100S, and P50S groups, respectively. When evidence of microorganisms’ growth was observed, PDT regimens eliminated over 90% of microorganisms on dentures. This clinical study showed that PDT was effective for disinfecting dentures.