Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera: Culicidae).
Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera: Culicidae).
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对按蚊种群（双翅目：蚊科）的核糖体 DNA 内转录间隔区 2 进行序列分析。
DOI：
10.1093/oxfordjournals.molbev.a040122
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发表时间：
1994
影响因子：
10.7
通讯作者：
Seawright,J
中科院分区：
文献类型：
--
作者：
Fritz,GN;Conn,J;Cockburn,A;Seawright,J
对在哥伦比亚、委内瑞拉、玻利维亚、苏里南和巴西收集的疟疾病媒努内兹托瓦按蚊群体的核糖体 DNA 内转录间隔区 2 (ITS2) 的序列变异进行了检查。来自哥伦比亚和委内瑞拉的蚊子具有相同的 ITS2 序列，并通过 3 个插入/缺失事件 (indels) 和 1 个颠换与其他种群的序列区分开来。 ITS2的长度为363-369 bp，G+C含量为55.3%-55.7%。群体之间和群体内部 ITS2 长度的变化是由于简单重复中的插入缺失造成的。来自以下三组的样本的 ITS2 共有序列相似或相同：（1）哥伦比亚、玻利维亚和委内瑞拉； (2) 苏里南和巴西北部； (3) 巴西东部和中部。巴西马瑙斯附近一个地点存在两个不同的共有序列，表明来自巴西东部和苏里南的种群聚集在亚马逊盆地的这个地区。这些数据表明，假定的隐匿物种 An. nuneztovari 的特点是 ITS2 区域 DNA 序列存在非常微小的差异。
Sequence variation of the ribosomal DNA internal transcribed spacer 2 (ITS2) was examined for populations of the malaria vector Anopheles nuneztovari collected in Colombia, Venezuela, Bolivia, Suriname, and Brazil. Mosquitoes from Colombia and Venezuela had identical ITS2 sequences and were distinguished from sequences in other populations by three insertion/deletion events (indels) and by one transversion. The length of the ITS2 was 363-369 bp, and it had a G+C content of 55.3%-55.7%. Variation in the length of the ITS2 between and within populations was due to indels in simple repeats. ITS2 consensus sequences were similar or identical for samples from the following three groups: (1) Colombia, Bolivia, and Venezuela; (2) Suriname and northern Brazil; and (3) eastern and central Brazil. The presence of two different consensus sequences from a single location near Manaus, Brazil, suggests that populations from eastern Brazil and those from Suriname converge in this region of the Amazon Basin. These data show that putative cryptic species of An. nuneztovari are distinguished by very minor differences in DNA sequence of the ITS2 region.