Antioxidant and hepatoprotective activity of vitex honey against paracetamol induced liver damage in mice
Antioxidant and hepatoprotective activity of vitex honey against paracetamol induced liver damage in mice
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牡荆蜂蜜对扑热息痛引起的小鼠肝损伤的抗氧化和保肝活性
DOI：
10.1039/c5fo00345h
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发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Sun, Liping
中科院分区：
文献类型：
--
作者：
Wang, Yuan;Li, Dan;Sun, Liping
研究了中国产的14种荆条蜂蜜对扑热息痛所致肝损伤的抗氧化和保肝作用。所有蜂蜜样品均具有较高的总酚含量(344~520 mg GAE/kg)、总黄酮类含量(19~31 mg/kg芦丁)和较强的清除DPPH自由基的抗氧化活性、铁还原抗氧化力和亚铁离子螯合能力。从荆条蜂蜜样品中检出9种酚酸，其中咖啡酸是主要成分。给小鼠灌胃给药(5g kg(-1)、20g kg(-1))70d，抗氧化活性以河北赞皇蜂蜜最高。与对乙酰氨基酚中毒组比较，高剂量(20g·kg~(-1))黄荆蜂蜜预处理组大鼠血清氧自由基吸收能力显著增强(15.07%)，Cu2+介导的脂蛋白氧化能力降低(80.07%)，丙氨酸转氨酶(75.79%)和天冬氨酸转氨酶(74.52%)受到抑制，超氧化物歧化酶和谷胱甘肽过氧化物酶活性增强，丙二醛(36.15%)和8-羟基-2‘-脱氧鸟苷(19.6%)生成减少。结果表明，荆条蜂蜜对扑热息痛所致肝损伤的保护作用可能与其抗氧化和/或氧化作用有关。
Fourteen vitex honeys from China were investigated to evaluate its antioxidant and hepatoprotective activity against paracetamol-induced liver damage. All honey samples exhibited high total phenolic content (344-520 mg GAE per kg), total flavonoid content (19-31 mg Rutin per kg), and strong antioxidant activity in DPPH radical scavenging, ferric reducing antioxidant power and Ferrous ion-chelating ability. Nine phenolic acids were detected in vitex honey samples, in which caffeic acid was the main compound. Honey from Heibei Zanhuang (S2) ranked the highest antioxidant activity was orally administered to mice (5 g kg(-1), 20 g kg(-1)) for 70 days. In high-dose (20 g kg(-1)), vitex honey pretreatment resulting in significant increase in serum oxygen radical absorbance capacity (15.07%) and decrease in Cu2+-mediate lipoprotein oxidation (80.07%), and suppression in alanine aminotransferase (75.79%) and aspartate aminotransferase (74.52%), enhancement in the superoxide dismutase and glutathione peroxidase activities and reduction in malondialdehyde (36.15%) and 8-hydroxy-2'-deoxyguanosine (19.6%) formation compared with paracetamol-intoxicated group. The results demonstrated the hepatoprotection of vitex honey against paracetamol-induced liver damage might attribute to its antioxidant and/or perhaps prooxidative property.