Induction of meiotic gynogenesis in yellow drum (Nibea albiflora, Sciaenidae) using heterologous sperm and evidence for female homogametic sex determination
Induction of meiotic gynogenesis in yellow drum (Nibea albiflora, Sciaenidae) using heterologous sperm and evidence for female homogametic sex determination
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DOI：
10.1016/j.aquaculture.2017.07.009
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发表时间：
2017-10-01
期刊：
影响因子：
4.5
通讯作者：
Liu, Feng
中科院分区：
文献类型：
--
作者：
Chen, Ruiyi;Lou, Bao;Liu, Feng
黄姑鱼（Nibea albiflora）是我国最重要的渔业资源之一，也是一种很有前途的水产养殖资源。这种鱼表现出显着的性别二态性增长和效率和盈利能力的水产养殖可以最大限度地提高所有女性的生产。因此，我们开发了一个有效的方案，诱导减数分裂雌核发育异源精子和性别决定系统的定义在黄鼓。采用条石鲷精子，确定精子遗传灭活的最佳紫外线照射剂量为45 mJ/cm（2）。对冷激温度、持续时间和引发时间进行了优化，最佳的雌核发育产量为受精后2 min，在4 ℃下冷激8 min。流式细胞仪DNA倍体检测结果表明，雌核发育幼虫为二倍体。微卫星位点的分析显示，在所有的雌核发育测试的独家母系遗传，进一步证实了成功的二倍体雌核发育，使用我们建立的协议。组织学检查的所有幼雌激素都是雌性，支持黄鼓的雌性同配性别决定的假设。因此，我们建立了一种有效、可靠的诱导黄背飞虱减数分裂雌核发育的方法。该方法可用于大规模生产单性黄姑鱼，以满足水产养殖业的需求。
The yellow drum (Nibea albiflora) is one of the most important species for commercial fisheries and a promising candidate for aquaculture in China. This fish exhibits significant sexually dimorphic growth and the efficiency and profitability of its aquaculture could be maximized by all-female production. Consequently, we developed an effective protocol to induce meiotic gynogenesis using heterologous sperm and defined the sex determination system in yellow drum. Spermatozoa from the rock bream (Oplegnathus fasciatus) were used, and the optimal UV-irradiation dose for genetically inactivating the spermatozoa was determined to be 45 mJ/cm(2). Cold-shock temperature, duration and time of initiation were optimized, and the best yield of gynogens was obtained with a cold-shock at 4 degrees C for a period of 8 min, initiated 2 min after fertilization. The DNA ploidy test by flow cytometry showed that the gynogenetic larvae were diploids. The analysis of microsatellite loci revealed an exclusive maternal inheritance in all the gynogens tested, further confirming the success of the diploid gynogenesis using the protocol that we established. All the juvenile gynogens examined by histology were female, supporting the assumption of female homogametic sex determination in the yellow drum. In conclusion, we have developed an effective and reliable method to induce meiotic gynogenesis of yellow drum. Our approach could be potentially applied to produce monosex yellow drum on a large scale to meet the demands of the aquaculture industry.