Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor.
Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor.
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DOI：
10.1038/srep27735
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发表时间：
2016-06-13
影响因子：
4.6
通讯作者：
Becher P
中科院分区：
文献类型：
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作者：
Postel A;Hansmann F;Baechlein C;Fischer N;Alawi M;Grundhoff A;Derking S;Tenhündfeld J;Pfankuche VM;Herder V;Baumgärtner W;Wendt M;Becher P
瘟病毒是属于持续生长的黄病毒科的高度可变的RNA病毒。最近在美国发现了一种遗传上非常独特的瘟病毒，命名为非典型猪瘟病毒（APPV）。在此，对369份来自表面健康成年猪的血清进行的筛查证实了德国存在APPV，估计个体患病率为2.4%，农场水平约为10%。此外，在患有先天性震颤（CT）的新生仔猪中检测到APPV基因组，但在未受影响的仔猪中不存在基因组。在腺上皮细胞、淋巴器官的滤泡中心、小脑的内颗粒细胞层以及三叉神经和脊神经节中鉴定出高负荷的基因组。对2007年小脑样本的回顾性分析表明，在CT未解决病因的仔猪中可以发现APPV。对欧洲第一个APPV多蛋白全编码序列的测定表明，与美国APPV序列的核苷酸同源性为88.2%。来自德国不同地区的APPV序列表现出高度可变性。总之，本研究的结果强烈表明，新生仔猪中存在的APPV基因组与CT相关，而在病毒血症成年猪中未观察到与临床疾病相关。
Pestiviruses are highly variable RNA viruses belonging to the continuously growing family Flaviviridae. A genetically very distinct pestivirus was recently discovered in the USA, designated atypical porcine pestivirus (APPV). Here, a screening of 369 sera from apparently healthy adult pigs demonstrated the existence of APPV in Germany with an estimated individual prevalence of 2.4% and ~10% at farm level. Additionally, APPV genomes were detected in newborn piglets affected by congenital tremor (CT), but genomes were absent in unaffected piglets. High loads of genomes were identified in glandular epithelial cells, follicular centers of lymphoid organs, the inner granular cell layer of the cerebellum, as well as in the trigeminal and spinal ganglia. Retrospective analysis of cerebellum samples from 2007 demonstrated that APPV can be found in piglets with CT of unsolved aetiology. Determination of the first European APPV complete polyprotein coding sequence revealed 88.2% nucleotide identity to the APPV sequence from the USA. APPV sequences derived from different regions in Germany demonstrated to be highly variable. Taken together, the results of this study strongly suggest that the presence of APPV genomes in newborn piglets correlates with CT, while no association with clinical disease could be observed in viremic adult pigs.