Diversity of CTX-M-positive Escherichia coli recovered from animals in Canada
Diversity of CTX-M-positive Escherichia coli recovered from animals in Canada
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DOI：
10.1016/j.vetmic.2019.02.031
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Boerlin, Patrick
中科院分区：
文献类型：
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作者：
Cormier, Ashley;Zhang, Pauline L. C.;Boerlin, Patrick
从历史上看，加拿大动物细菌中的超广谱头孢菌素耐药性归因于SHV和CMY β-内酰胺酶家族。随着bla（CTX-M）基因家族在从各种动物物种回收的大肠杆菌中的出现，这种模式开始改变。本文对bla（CTX-M）阳性大肠杆菌的全基因组序列进行了分析和比较。从加拿大的狗、鸡、猪、马和肉牛中分离的螺旋体分离物（n = 173）。鉴定了10种bla（CTX-M）变体，其中在大多数动物物种中鉴定了bla（CTX-M-1、-14、-15、-27）和bla（CTX-M-55）。这些变异发生在许多序列类型中，表明移动的遗传元件介导bla（CTX-M）的传播。变体bla（CTX-M-14、-15、-27）和bla（CTXM-55）与bla（CTX-M）在人类临床分离株中的全球传播相关，并且它们的存在可能指示人类和动物之间的转移。这些变异体也是在序列类型131分离株中鉴定的主要变异体，这些分离株与犬以外的任何其他物种无关。这些分离株携带与大肠杆菌相同的bla（CTX-M）变异体。在人体内发现的大肠杆菌。密切接触可能会促进这些分离株在人类和伴侣动物之间的传播。
Historically, extended-spectrum cephalosporin resistance in bacteria from animals in Canada has been attributed to the SHV and CMY beta-lactamase families. This pattern is beginning to change with the emergence of the bla(CTX-M) gene family among Escherichia coil recovered from various animal species. Here we analyze and compare whole genome sequences of bla(CTX-M)-positive E. coil isolates (n = 173) from dogs, chicken, swine, horses and beef cattle in Canada. Ten bla(CTX-M) variants were identified with bla(CTX-M-1,-14, -15, -27) and bla(CTX-M-55) being identified in most animal species. These variants occurred across many sequence types, suggesting that mobile genetic elements mediate the spread of bla(CTX-M). The variants bla(CTX-M-14,-15,-27) and bla(CTXM-55) are associated with the global spread of bla(CTX-M) in human clinical isolates and their presence could be indicative of transfer between humans and animals. These variants were also the principal variants identified among sequence type 131 isolates, which were not associated with any other species than dogs. These isolates carried the same bla(CTX-M) variants as E. coli isolates found in humans. Close contact may promote the transmission of these isolates between humans and companion animals.