Tissue factor modulates the thrombogenicity of human atherosclerotic plaques
Tissue factor modulates the thrombogenicity of human atherosclerotic plaques
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DOI：
10.1161/01.cir.95.3.594
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发表时间：
1997-02-04
期刊：
影响因子：
37.8
通讯作者：
Badimon, JJ
中科院分区：
文献类型：
--
作者：
Toschi, V;Gallo, R;Badimon, JJ
背景：动脉粥样硬化破裂病变的血栓形成取决于斑块成分暴露于流动血液的性质和程度，以及局部流变学和各种全身因素。我们以前曾报道过不同类型的人类动脉粥样硬化病变的不同血栓形成时，暴露于流动的血液在一个良好的特点灌注系统。本研究探讨组织因子的作用，在不同类型的动脉粥样硬化斑块及其component.Methods和结果的血栓形成的人动脉段（5泡沫细胞丰富，9胶原丰富，10脂质丰富的动脉粥样硬化病变和26个正常，nonatherosclerotic段）暴露于肝素化的血液在高剪切速率条件下的Badimon灌注室。用In-111标记的血小板评价动脉标本的致血栓性。灌注后，通过使用因子VIIa的原位结合试验对标本进行组织因子染色。样本中的组织因子以0至3的量表进行半定量评估。富含脂质的动脉粥样硬化核心上的血小板沉积显著高于所有其他基质（P = .0002）。与其他动脉组分相比，富脂核心也显示出最强的组织因子染色（3+/-0.1任意单位）。所有标本的比较显示，血小板沉积定量与组织因子染色评分呈正相关（r= 0.35，P
Background The thrombogenicity of a disrupted atherosclerotic lesion is dependent on the nature and extent of the plaque components exposed to flowing blood together with local rheology and a variety of systemic factors. We previously reported on the different thrombogenicity of the various types of human atherosclerotic lesions when exposed to flowing blood in a well-characterized perfusion system. This study examines the role of tissue factor in the thrombogenicity of different types of atherosclerotic plaques and their components.Methods and Results Fifty human arterial segments (5 foam cell-rich, 9 collagen-rich, and 10 lipid-rich atherosclerotic lesions and 26 normal, nonatherosclerotic segments) were exposed to heparinized blood at high shear rate conditions in the Badimon perfusion chamber. The thrombogenicity of the arterial specimens was assessed by In-111-labeled platelets. After perfusion, specimens were stained for tissue factor by use of an in situ binding assay for factor VIIa. Tissue factor in specimens was semiquantitatively assessed on a scale of 0 to 3. Platelet deposition on the lipid-rich atheromatous core was significantly higher than on all other substrates (P = .0002). The lipid-rich core also exhibited the most intense tissue factor staining (3+/-0.1 arbitrary units) compared with other arterial components. Comparison of all specimens showed a positive correlation between quantitative platelet deposition and tissue factor staining score (r=.35, P