DIFFERENTIAL RELEASE OF HISTAMINE AND EICOSANOIDS FROM HUMAN-SKIN MAST-CELLS ACTIVATED BY IGE-DEPENDENT AND NON-IMMUNOLOGICAL STIMULI
DIFFERENTIAL RELEASE OF HISTAMINE AND EICOSANOIDS FROM HUMAN-SKIN MAST-CELLS ACTIVATED BY IGE-DEPENDENT AND NON-IMMUNOLOGICAL STIMULI
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DOI：
10.1111/j.1476-5381.1989.tb12030.x
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发表时间：
1989-07-01
影响因子：
7.3
通讯作者：
CHURCH, MK
中科院分区：
文献类型：
--
作者：
BENYON, RC;ROBINSON, C;CHURCH, MK
使用胶原酶和透明质酸酶的混合物将细胞从人包皮中分散，并通过密度梯度离心分离成肥大细胞耗尽（75%）的制剂。用ε-链特异性抗人IgE进行梯度级分的挑战刺激了组胺、前列腺素D2(PGD2)和白三烯C4(LTC4)的释放。类二十烷酸的释放与组胺的释放显着相关，表明它们源自分散物的肥大细胞群。在高度纯化的(76.2±4.2％)肥大细胞制剂中，组胺、PGD2和LTC4的最大净释放量为3432±。 725, 84.9 .+-. 10.8 和 6.6.+-。 1.2 pmol/106 个有核细胞。非免疫刺激物质 P、血管活性肠肽 (VIP)、生长抑素、化合物 48/80、吗啡和聚 L-赖氨酸释放的组胺量与抗 IgE 相似，但 PGD2 和 LTC4 少 12 至 21 倍。这些研究表明，IgE 依赖性和非免疫刺激通过不同的分泌机制激活人类皮肤肥大细胞，这一假设得到了我们之前关于 Ca2+ 需求差异和组胺释放时间进程的发现的支持。由于皮肤肥大细胞和含有神经肽的真皮神经末梢之间的密切解剖学关联，非免疫机制的激活在体内可能很重要。
Cells were dispersed from human foreskin using a mixture of collagenase and hyaluronidase and separated into mast cell-depleted (75%) preparations by density-gradient centrifugation. Challenge of gradient fractions with .epsilon.-chain-specific anti-human IgE stimulated the release of histamine, prostaglandin D2 (PGD2) and leukotriene C4 (LTC4). The release of eicosanoids was significantly correlated with that of histamine, suggesting that they are derived from the mast cell population of the dispersate. In highly purified (76.2 .+-. 4.2%) mast cell preparations, maximum net release of histamine, PGD2 and LTC4 was 3432 .+-. 725, 84.9 .+-. 10.8 and 6.6 .+-. 1.2 pmol/106 nucleated cells. The non-immunological stimuli substance P, vasoactive intestinal peptide (VIP), somatostatin, compound 48/80, morphine and poly-L-lysine released similar amounts of histamine to anti-IgE, but 12 to 21 fold less PGD2 and LTC4. These studies suggest that IgE-dependent and non-immunological stimuli activate human skin mast cells by different secretory mechanisms, a hypothesis supported by our previous findings of differences in Ca2+ requirements and time-course of histamine release. Activation by the nonimmunological mechanism may be of importance in vivo due to the close anatomical association between skin mast cells and dermal nerve-terminals containing neuropeptides.