THE LEMA GENE REQUIRED FOR PATHOGENICITY OF PSEUDOMONAS-SYRINGAE PV SYRINGAE ON BEAN IS A MEMBER OF A FAMILY OF 2-COMPONENT REGULATORS
THE LEMA GENE REQUIRED FOR PATHOGENICITY OF PSEUDOMONAS-SYRINGAE PV SYRINGAE ON BEAN IS A MEMBER OF A FAMILY OF 2-COMPONENT REGULATORS
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DOI：
10.1128/jb.174.9.3011-3020.1992
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发表时间：
1992-05-01
影响因子：
3.2
通讯作者：
WILLIS, DK
中科院分区：
文献类型：
--
作者：
HRABAK, EM;WILLIS, DK
植物病原体丁香假单胞菌 pv. 的 lemA 基因。豆科植物病斑的形成需要丁香菌。先前分离了反式补充lemA突变体的粘粒克隆。 lemA 基因通过亚克隆和转座子诱变进行定位。对 lemA 区域和侧翼 DNA 进行了测序，并鉴定了 2.7 kb 的开放阅读框。 lemA 基因的核苷酸和预测氨基酸序列显示出与原核双组分调节蛋白家族的序列相似性。与之前描述的大多数双组分系统不同，lemA 基因产物与一种蛋白质中的两种组分具有同源性。 lemA 基因上游和下游引入的突变未能定位第二个蛋白质成分的基因，但鉴定出了丁香假单胞菌 pv 的推定 cysM 基因。丁香科。 cysM 基因位于 lemA 基因的上游，并以发散方式转录。 lemA 基因产物在丁香假单胞菌 pv. 中低水平表达。丁香菌并且似乎是积极的自我调节。
The lemA gene of the plant pathogen Pseudomonas syringae pv. syringae is required for disease lesion formation on bean plants. Cosmid clones that complemented a lemA mutant in trans were isolated previously. The lemA gene was localized by subcloning and transposon mutagenesis. The lemA region and flanking DNA were sequenced, and an open reading frame of 2.7 kb was identified. The nucleotide and predicted amino acid sequences of the lemA gene showed sequence similarity to a family of prokaryotic two-component regulatory proteins. Unlike most of the previously described two-component systems, the lemA gene product contained homology to both components in one protein. Mutations introduced upstream and downstream of the lemA gene failed to locate a gene for a second protein component but identified the putative cysM gene of P. syringae pv. syringae. The cysM gene was located upstream of the lemA gene and was divergently transcribed. The lemA gene product was expressed at low levels in P. syringae pv. syringae and appeared to be positively autoregulated.