The biofilm architecture of sixty opportunistic pathogens deciphered using a high throughput CLSM method
The biofilm architecture of sixty opportunistic pathogens deciphered using a high throughput CLSM method
复制标题
DOI：
10.1016/j.mimet.2010.04.006
复制
发表时间：
2010-07-01
影响因子：
2.2
通讯作者：
Briandet, R.
中科院分区：
文献类型：
--
作者：
Bridier, A.;Dubois-Brissonnet, F.;Briandet, R.
本研究提出了一种基于共聚焦激光扫描显微镜（CLSM）的高通量方法，结合使用96-威尔斯微孔板兼容高分辨率成像的生物膜形成和结构的研究。作为例证，由此观察并量化了由60种条件致病菌形成的生物膜的三维结构。结果显示了生物膜结构的多样性。观察到特定的空间排列，如铜绿假单胞菌已经描述的蘑菇样结构。其他功能，如肠道沙门氏菌菌株Agona的小菌落中的中空空隙，首次被确定。结合使用微孔板和共聚焦成像被证明是一个很好的替代其他高通量的方法，通常使用，因为它能够直接，原位，定性和定量表征生物膜结构。这种高含量的方法应该导致更清楚地了解生物膜性状中所涉及的结构-功能关系。(C)2010 Elsevier B. V.保留所有权利。
This study proposes a high throughput method based on Confocal Laser Scanning Microscopy (CLSM) combined with the use of 96-wells microtiter plates compatible with high resolution imaging for the study of biofilm formation and structure. As an illustration, the three-dimensional structures of biofilms formed by 60 opportunistic pathogens were thus observed and quantified. The results revealed the diversity of biofilm architectures. Specific spatial arrangement such as the mushroom-like structures already described for Pseudomonas aeruginosa was observed. Other features, such as hollow voids in microcolonies of Salmonella enterica strain Agona, were identified for the first time. The combined use of microplates and confocal imaging proved to be a good alternative to the other high throughput methods commonly used as it enables the direct, in situ, qualitative and quantitative characterization of biofilm architecture. This high content method should lead to a clearer understanding of the structure-function relationships implicated in biofilms traits. (C) 2010 Elsevier B.V. All rights reserved.