Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments
Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments
复制标题
DOI：
10.1002/adhm.201700052
复制
发表时间：
2017-08-09
影响因子：
10
通讯作者：
Salmeron-Sanchez, Manuel
中科院分区：
文献类型：
--
作者：
Cipitria, Amaia;Salmeron-Sanchez, Manuel
工程细胞微环境涉及生化因子，细胞外基质（ECM）和与邻近细胞的相互作用。这份进展报告提供了一个关键的研究，将生长因子（GF）的信号和mechanotransduction到先进的微环境的设计的关键概述。已经开发了用于GF的表面结合呈递的材料系统，其通过与基质的物理化学亲和力共价束缚或螯合，作为可溶性GF的替代物。此外，一些材料含有GF和整联蛋白结合区，从而使两者之间的协同信号传导成为可能。机械转导是指细胞感知ECM的物理性质并将其转化为生化信号的能力。ECM的物理学的各个方面，即刚度，几何形状和配体间距，以及时间依赖性的属性，如矩阵硬化，降解性，粘弹性，表面流动性以及空间图案和梯度的物理线索进行了讨论。总而言之，各种例子说明了生长因子协同信号传导的潜力和微环境的物理特性，可用于再生医学、癌症研究和药物测试。
Engineering cellular microenvironments involves biochemical factors, the extracellular matrix (ECM) and the interaction with neighbouring cells. This progress report provides a critical overview of key studies that incorporate growth factor (GF) signalling and mechanotransduction into the design of advanced microenvironments. Materials systems have been developed for surface-bound presentation of GFs, either covalently tethered or sequestered through physico-chemical affinity to the matrix, as an alternative to soluble GFs. Furthermore, some materials contain both GF and integrin binding regions and thereby enable synergistic signalling between the two. Mechanotransduction refers to the ability of the cells to sense physical properties of the ECM and to transduce them into biochemical signals. Various aspects of the physics of the ECM, i.e. stiffness, geometry and ligand spacing, as well as time-dependent properties, such as matrix stiffening, degradability, viscoelasticity, surface mobility as well as spatial patterns and gradients of physical cues are discussed. To conclude, various examples illustrate the potential for cooperative signalling of growth factors and the physical properties of the microenvironment for potential applications in regenerative medicine, cancer research and drug testing.