Overexpression of an antioxidant enzyme improves male mating performance after stress in a lek-mating fruit fly
Overexpression of an antioxidant enzyme improves male mating performance after stress in a lek-mating fruit fly
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抗氧化酶的过度表达可改善莱克交配果蝇应激后的雄性交配表现
DOI：
10.1098/rspb.2019.0531
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Hahn, Daniel A.
中科院分区：
文献类型：
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作者：
Teets, Nicholas M.;Dias, Vanessa S.;Pierce, Bailey K.;Schetelig, Marc F.;Handler, Alfred M.;Hahn, Daniel A.
在许多物种中，求偶展示是雄性质量的可靠信号，目前的假设表明，这些展示允许雌性选择具有高细胞功能的雄性。环境应激源产生过量的活性氧（ROS），损害细胞功能，因此清除ROS的抗氧化途径可能对保持复杂的性行为至关重要。在这里，我们测试的假设，增强线粒体中的抗氧化活性保护交配性能后氧化应激。使用转基因的方法，我们直接操纵线粒体的抗氧化活性在加勒比海果蝇，Anastrepha suspensa，一个lek交配物种与精心制作的性展示和激烈的性选择，也是不育昆虫技术方案的模型。我们产生了7个转基因株系，过表达线粒体超氧化物歧化酶（MnSOD）。辐射是一种严重的氧化应激源，用于诱导昆虫不育。辐射处理后，两个线与中间MnSOD过表达表现出增强交配性能相对于野生型男性。这些改善交配对应于减少脂质的氧化损伤，表明MnSOD过表达保护苍蝇在细胞水平上的氧化应激。对于具有改善的交配性能的品系，过表达也保留了运动活性，如实验室攀爬测定所示。我们的研究结果表明，氧化应激，抗氧化能力和男性性能之间存在明显的联系。我们的工作具有从根本上理解抗氧化剂在性选择中的作用的影响，并显示出使用转基因方法来提高昆虫的田间表现，释放区域范围内的害虫管理策略和提高生物控制剂的性能一般的承诺。
In many species, courtship displays are reliable signals of male quality, and current hypotheses suggest that these displays allow females to choose males with high cellular function. Environmental stressors generate excess reactive oxygen species (ROS) that impair cellular function, and thus antioxidant pathways that remove ROS are probably critical for preserving complex sexual behaviours. Here, we test the hypothesis that enhanced antioxidant activity in mitochondria preserves mating performance following oxidative stress. Using a transgenic approach, we directly manipulated mitochondrial antioxidant activity in the Caribbean fruit fly,Anastrepha suspensa,a lek-mating species with elaborate sexual displays and intense sexual selection that is also a model for sterile insect technique programmes. We generated seven transgenic lines that overexpress mitochondrial superoxide dismutase (MnSOD). Radiation is a severe oxidative stressor used to induce sterility for sterile insect programmes. After radiation treatment, two lines with intermediate MnSOD overexpression showed enhanced mating performance relative to wild-type males. These improvements in mating corresponded with reduced oxidative damage to lipids, demonstrating that MnSOD overexpression protects flies from oxidative stress at the cellular level. For lines with improved mating performance, overexpression also preserved locomotor activity, as indicated by a laboratory climbing assay. Our results show a clear link between oxidative stress, antioxidant capacity and male performance. Our work has implications for fundamentally understanding the role of antioxidants in sexual selection, and shows promise for using transgenic approaches to enhance the field performance of insects released for area-wide pest management strategies and improving performance of biological control agents in general.