Th1: Mediator Lymphocytes in Experimental Autoimmune Labyrinthitis
Th1: Mediator Lymphocytes in Experimental Autoimmune Labyrinthitis
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Th1：实验性自身免疫性迷路炎中的介导淋巴细胞
DOI：
10.1080/00016480152583601
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发表时间：
2001
影响因子：
1.4
通讯作者：
S. Tomiyama
中科院分区：
文献类型：
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作者：
S. Tomiyama
先前对实验性自身免疫性迷路炎（EAL）的研究一致表明，淋巴细胞仅短暂浸润至小鼠内耳。为了阐明 EAL 启动时淋巴细胞的概况，本研究使用免疫组织化学技术研究了从第 4 天到第 35 天的细胞表面抗原以及细胞因子。许多CD4+细胞早在第4天就主要浸润内淋巴囊，并逐渐扩散到内耳的其余部分。尽管细胞数量逐渐减少，但浸润在第 12 天达到顶峰，并且在大多数动物中持续到第 35 天。相比之下，第10天，所有动物的内耳中都发现很少有CD8+细胞出现，并且细胞数量迅速减少。第 4 天在内淋巴囊中鉴定出许多 IFN-n 和 IL-2 阳性细胞。这些结果表明辅助 T1 淋巴细胞，而不是细胞毒性 T 淋巴细胞，可能在 EAL 的启动中发挥核心作用。
A previous study on experimental autoimmune labyrinthitis (EAL) consistently demonstrated transient infiltration of lymphocytes only into the inner ear of mice. To clarify the profile of lymphocytes in the initiation of EAL, the present study investigated cell surface antigens, as well as cytokines, from Day 4 to Day 35, using immunohistochemical techniques. Many CD4+ cells mainly infiltrated the endolymphatic sac as early as Day 4 and gradually spread to the rest of the inner ear. Infiltration peaked on Day 12 and persisted in most animals until Day 35, although the number of cells gradually decreased. In contrast, very few CD8+ cells were found to have appeared in the inner ear of all animals on Day 10, and the number of cells rapidly decreased. Many cells positive for IFN- n and IL-2 were identified in the endolymphatic sac on Day 4. These results suggest that helper T1 lymphocytes, rather than cytotoxic T lymphocytes, may play a central role in the initiation of EAL.