Amperometric enzyme electrode for glucose determination based on poly(pyrrole-2-aminobenzoic acid)
Amperometric enzyme electrode for glucose determination based on poly(pyrrole-2-aminobenzoic acid)
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DOI：
10.1007/s10008-009-0895-1
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发表时间：
2010-06
影响因子：
2.5
通讯作者：
A. Berkkan;Ali İhsan Seçkin;K. Pekmez;U. Tamer
中科院分区：
文献类型：
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作者：
A. Berkkan;Ali İhsan Seçkin;K. Pekmez;U. Tamer
研究了吡咯与2-氨基苯甲酸的聚合反应，在铂电极上电化学得到了稳定的聚吡咯-2-氨基苯甲酸功能化膜(PP2ABA)。聚吡咯和PP2ABA在电合成过程中表现出不同的循环伏安行为。傅立叶变换红外光谱和表面增强拉曼光谱测试证实了两种薄膜中含有羧酸基。葡萄糖氧化酶被共价固定在导电的PP2ABA膜上，在pH=6.2时，在0.1M磷酸盐缓冲液中，在0.7nV与Ag/AgCl2的电位下，测量了葡萄糖浓度的函数关系。考察了聚合膜厚度、pH和可能的干扰因素对测定结果的影响。校准曲线的线性范围为3~40 mm，灵敏度为0.058µA/mm−1 cm−2，检出限为0.5 mm。经计算，表观米氏常数KM为1 × 10−2 mm，响应时间为S的5。
Polymerization of pyrrole and 2-aminobenzoic acid has been investigated, and a functionalized stable film of poly(pyrrole-2-aminobenzoic acid) (PP2ABA) has been obtained electrochemically onto platinum electrode. Different cyclic voltammetric behavior is obtained for polypyrrole and PP2ABA during electrosynthesis. Fourier-transformed infrared spectrometry and surface-enhanced Raman spectrometry measurements on the two films have confirmed the presence of carboxylate group in the films. The enzyme, glucose oxidase, was covalently immobilized on a conducting PP2ABA film, and amperometric response was measured as a function of concentration of glucose at a potential of 0.7 V vs Ag/AgCl in 0.1 M phosphate buffer at pH 6.2. The effect of polymeric film thickness, pH, and possible interferents were investigated. The linear range of the calibration curve is from 3 to 40 mM with a sensitivity of 0.058 µA mM−1cm−2and a limit of detection of 0.5 mM. The apperent Mishaelis–Menten constantKMis calculated to be 1 × 10−2mM, and the response time is 5 s.