INFLUENCE OF CHARGE ON FILTRATION ACROSS RENAL BASEMENT-MEMBRANE FILMS INVITRO
INFLUENCE OF CHARGE ON FILTRATION ACROSS RENAL BASEMENT-MEMBRANE FILMS INVITRO
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DOI：
10.1038/ki.1984.49
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发表时间：
1984-01-01
影响因子：
19.6
通讯作者：
ROBINSON, GB
中科院分区：
文献类型：
--
作者：
BRAY, J;ROBINSON, GB
不同带电荷的肌红蛋白和葡聚糖在离体基底膜上的过滤表明，这两种聚合物的过滤行为都受到电荷的影响。拒收率随负电荷的增加而增加。分离基膜的滴定结果显示，等离子点pH为5.5，等电点pH为5.7。pH 7.4时净负电荷为0.15 mEq/g蛋白；这种电荷归因于羧酸阴离子。糖胺聚糖对净电荷无显著影响。在基底膜的等电点上，窄范围葡聚糖组分的过滤明显减少了由于电荷导致的过滤差异，这证实了在pH 7.4下发现的行为差异是由于溶质和膜之间的电荷相互作用造成的。对带电荷和不带电荷的葡聚糖组分的物理表征表明，不同带电荷的葡聚糖在大小和形状上没有实质性的差异。
The filtration of differently charged species of myoglobin and dextran across films of isolated basement membrane in vitro showed that the filtration behavior of both polymers was influenced by charge. Rejection increased with increasing negative charge. Titration of the isolated basement membrane revealed an isoionic point of pH 5.5 and an isoelectric point of pH 5.7. The net negative charge at pH 7.4 was 0.15 mEq/g protein; this charge was attributed to carboxylate anion. Glycosaminoglycan sulfate did not contribute significantly to the net charge. Filtration of narrow range dextran fractions across films of basement membrane at the isoelectric point markedly reduced differences in filtration due to charge confirming that the differences in behavior found at pH 7.4 were due to charge interactions between the solutes and the membranes. Physical characterization of the charged and uncharged dextran fractions revealed no substantial differences in size or shape for the differently charged species.