Col11a1 Regulates Bone Microarchitecture during Embryonic Development.
Col11a1 Regulates Bone Microarchitecture during Embryonic Development.
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DOI：
10.3390/jdb3040158
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发表时间：
2015
影响因子：
2.7
通讯作者：
Oxford JT
中科院分区：
文献类型：
--
作者：
Hafez A;Squires R;Pedracini A;Joshi A;Seegmiller RE;Oxford JT
胶原蛋白XIα1(COL11A1)是胚胎发育所必需的一种细胞外基质分子，在胶原蛋白纤维组装过程中既能促进纤维的形成，又能调节异型纤维的直径。虽然在脊椎动物体内的许多不同组织中都发现了COL11A1，但COL11A1在软骨内成骨过程中发挥着重要作用。为了进一步了解COL11A1在骨形成过程中的作用，我们利用X射线显微断层扫描(Micro-CT)和组织学方法比较了野生型(WT)小鼠和COL11A1缺陷小鼠的骨骼矿化情况。观察到骨小梁微结构的变化，并在此进行了描述。此外，还观察到发育中的长骨的骨膜骨领的变化，并导致COL11A1缺陷小鼠的厚度比WT仔鼠增加。在没有COL11A1的情况下，椎体形成不完整。这些数据表明，COL11A1的缺失会导致新生骨的改变，这与COL11A1在矿化中的作用是一致的。这些结果表明，COL11A1在生长板和软骨膜中的表达是软骨内成骨过程中骨小梁和骨领形成所必需的。观察到的矿化组织的变化进一步定义了COL11A1的功能。
Collagen XI alpha 1 (Col11a1) is an extracellular matrix molecule required for embryonic development with a role in both nucleating the formation of fibrils and regulating the diameter of heterotypic fibrils during collagen fibrillar assembly. Although found in many different tissues throughout the vertebrate body, Col11a1 plays an essential role in endochondral ossification. To further understand the function of Col11a1 in the process of bone formation, we compared skeletal mineralization in wild-type (WT) mice and Col11a1-deficient mice using X-ray microtomography (micro-CT) and histology. Changes in trabecular bone microstructure were observed and are presented here. Additionally, changes to the periosteal bone collar of developing long bones were observed and resulted in an increase in thickness in the case of Col11a1-deficient mice compared to WT littermates. Vertebral bodies were incompletely formed in the absence of Col11a1. The data demonstrate that Col11a1 depletion results in alteration to newly-formed bone and is consistent with a role for Col11a1 in mineralization. These findings indicate that expression of Col11a1 in the growth plate and perichondrium is essential for trabecular bone and bone collar formation during endochondral ossification. The observed changes to mineralized tissues further define the function of Col11a1.