dTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila.
dTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila.
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dTBI2：一种经过校准、可调谐的设备，用于治疗果蝇脑外伤。
DOI：
10.1002/cpz1.996
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Barber,AnnikaF
中科院分区：
文献类型：
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作者：
Ratner,StephenW;Fetchko,Michael;Mathivanan,AkankshaS;Kelly,SeannaE;Gupta,Shambhavi;Barber,AnnikaF
第二代果蝇非创伤性脑损伤（TBI）装置dTBI2通过提供中等通量、可调、头部特异性损伤来改善果蝇非创伤性脑损伤给药。我们更新的设备设计提高了用户友好性，消除了受伤时间的不一致，并具有更新的电路设计，以延长精密电子元件的使用寿命。dbi2提高了用户和运行的可重复性，并导致更一致的损伤后表型。该协议描述了dTBI2设备的构造，校准和使用，该设备使用Arduino控制的压电致动器来提供将苍蝇头压缩在金属项圈上的力。头部压缩的持续时间和深度是可调的，允许校准损伤的严重程度。所有设备组件都是市售的，整个设备可以在一周内以不到1000美元的价格建造完成。dTBI2的设计将使任何实验室都能够为果蝇atbi建立一个高度可靠、低成本的设备，促进果蝇闭头TBI的采用和探索，以进行前向遗传筛选。我们将在下面描述构建dbi2设备所需的三个协议。©2024作者。Wiley期刊有限责任公司发布的当前协议基本协议1:dTBI2控制装置的构造基本协议2：压电致动器外壳的构造基本协议3:dTBI2损伤的管理
The second‐generationDrosophilatraumatic brain injury (TBI) device dTBI2 improvesDrosophilaTBI administration by providing a moderate‐throughput, tunable, head‐specific injury. Our updated device design improves user‐friendliness, eliminates inconsistencies in injury timing, and has an updated circuit design to extend the longevity of delicate electronic components. dTBI2 improves reproducibility across users and runs, and results in more consistent post‐injury phenotypes. This protocol describes the construction, calibration, and use of the dTBI2 device, which uses an Arduino‐controlled piezoelectric actuator to deliver a force that compresses a fly head against a metal collar. The duration and depth of head compression is tunable, allowing calibration of injury severity. All device components are commercially available, and the entire device can be constructed in under a week for less than $1000. The dTBI2 design will enable any lab to build a highly reliable, low‐cost device forDrosophilaTBI, facilitating increased adoption and ease of exploration of closed‐head TBI inDrosophilafor forward genetic screens. We describe below the three protocols necessary for constructing a dTBI2 device. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Construction of the dTBI2 control deviceBasic Protocol 2: Construction of the piezoelectric actuator housingBasic Protocol 3: Administration of dTBI2 injuries