DIRECT AND RAPID DETECTION OF ERYSIPELOTHRIX-RHUSIOPATHIAE DNA IN ANIMALS BY PCR
DIRECT AND RAPID DETECTION OF ERYSIPELOTHRIX-RHUSIOPATHIAE DNA IN ANIMALS BY PCR
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DOI：
10.1128/jcm.32.6.1526-1531.1994
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发表时间：
1994-06-01
影响因子：
9.4
通讯作者：
MORITA, H
中科院分区：
文献类型：
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作者：
MAKINO, SI;OKADA, Y;MORITA, H
丹毒弧菌是一种革兰氏阳性杆状病毒，能引起猪丹毒。为了建立一种实际应用的特异性检测方法，如屠宰场检查，我们研究了在pcr特异性检测系统中使用编码16S rRNA的DNA序列衍生的引物的可行性。设计寡核苷酸引物，通过PCR扩增407 bp的DNA片段。扩增对丹毒杆菌的DNA有特异性，而对其他细菌属没有特异性。该方法在6 h内高效、特异地检测出小鼠关节和脾脏样本中的丹毒DNA序列，并应用细菌数量极低的样本(
Erysipelothrix rhusiopathiae is a gram-positive rod capable of causing erysipelas in swine. To establish a method for specifically detecting E. rhusiopathiae for practical applications, such as for the inspection of slaughterhouses, the feasibility of using primers derived from the DNA sequence coding for 16S rRNA in a PCR-specific detection system was investigated. Oligonucleotide primers were designed to amplify a 407-bp DNA fragment by PCR. The amplification was specific to the Erysipelothrix DNA but not to that of other bacterial genera tested. This PCR-based method efficiently and specifically detected the Erysipelothrix DNA sequence in joint and spleen samples from mice within 6 h, and application of the 407-bp DNA segment from samples containing very low numbers of bacteria (