Low temperature acclimation with electrical stimulation enhance the biocathode functioning stability for antibiotics detoxification
Low temperature acclimation with electrical stimulation enhance the biocathode functioning stability for antibiotics detoxification
复制标题
电刺激低温驯化增强抗生素解毒生物阴极功能稳定性
DOI：
10.1016/j.watres.2016.05.028
复制
发表时间：
2016
期刊：
影响因子：
12.8
通讯作者：
Wang Aijie
中科院分区：
文献类型：
--
作者：
Liang Bin;Kong Deyong;Ma Jincai;Wen Chongqing;Yuan Tong;Lee Duu-Jong;Zhou Jizhong;Wang Aijie
提高污水处理系统中功能微生物群落的稳定性是促进寒区污染物脱毒的关键。虽然生物阴极群落可以加速环境污染物的降解，但如何适应冷胁迫和提高功能微生物群落的催化稳定性仍然知之甚少。在此，我们研究了抗生素氯霉素（CAP）对生物阴极群落的结构和功能的响应，包括恒定低温10°C （10-biocathode）和温度从10°C升高到25°C （S25-biocathode）。结果表明，电刺激低温驯化在开路和闭路条件下，对芳香胺产物amcl2的生成效率与10-生物阴极和s25 -生物阴极进行比较，发现电刺激低温驯化明显提高了CAP硝基还原效率。10-生物阴极产生的AMCl比s25 -生物阴极最大，但AMCl对AMCl的脱卤率显著低于s25 -生物阴极。持续低温和温度升高均使10-生物阴极和s25 -生物阴极的核心功能群落富集。10-生物阴极的功能稳定性主要通过选择性富集冷适应功能物种、代谢相似的硝基芳烃还原剂共存以及维持关键电子转移基因的相对丰度来维持。该研究为加速冷废水系统中环境污染物降解的生物阴极功能稳定性提供了新的见解。
Improvement of the stability of functional microbial communities in wastewater treatment system is critical to accelerate pollutants detoxification in cold regions. Although biocathode communities could accelerate environmental pollutants degradation, how to acclimate the cold stress and to improve the catalytic stability of functional microbial communities are remain poorly understood. Here we investigated the structural and functional responses of antibiotic chloramphenicol (CAP) reducing biocathode communities to constant low temperature 10 °C (10-biocathode) and temperature elevation from 10 °C to 25 °C (S25-biocathode). Our results indicated that the low temperature acclimation with electrical stimulation obviously enhanced the CAP nitro group reduction efficiency when comparing the aromatic amine product AMCl2formation efficiency with the 10-biocathode and S25-biocathode under the opened and closed circuit conditions. The 10-biocathode generated comparative AMCl maximum as the S25-biocathode but showed significant lower dehalogenation rate of AMCl2to AMCl. The continuous low temperature and temperature elevation both enriched core functional community in the 10-biocathode and S25-biocathode, respectively. The 10-biocathode functioning stability maintained mainly through selectively enriching cold-adapted functional species, coexisting metabolically similar nitroaromatics reducers and maintaining the relative abundance of key electrons transfer genes. This study provides new insights into biocathode functioning stability for accelerating environmental pollutants degradation in cold wastewater system.