Interferon-gamma induction of major histocompatibility complex antigens on cultured bovine luteal cells.
Interferon-gamma induction of major histocompatibility complex antigens on cultured bovine luteal cells.
复制标题
在培养的牛黄体细胞上干扰素-γ诱导主要组织相容性复合物抗原。
作者：
D. L. Fairchild;J. Pate
为了研究可能参与黄体功能的免疫学机制，研究了培养的牛黄体细胞上I类和II类主要组织相容性复合物（MHC）抗原的存在。在无血清培养72小时后，I类抗原显着表达的黄体细胞，通过间接免疫荧光法测定，而II类抗原的表达是有限的。通过用T淋巴细胞因子干扰素-γ（IFN-γ）治疗，黄体细胞上MHC抗原的表达增加。I类和II类抗原分别升高25%和370%以上的控制，IFN-γ暴露后。由于黄体受促黄体激素（LH）调节，因此用单独的激素或除IFN-γ外的激素处理黄体细胞，并测定抗原表达。LH处理减弱了牛黄体细胞上II类抗原的IFN-γ诱导。这些观察结果是第一次证明牛黄体细胞上存在MHC抗原，以及淋巴因子IFN-γ和LH对抗原表达的调节。
To investigate immunological mechanisms that may be involved in luteal function, the presence of Class I and Class II major histocompatibility complex (MHC) antigens on cultured bovine luteal cells was examined. After 72 h in serum-free culture, Class I antigens were markedly expressed on luteal cells, as determined by indirect immunofluorescence, whereas expression of Class II antigens was limited. The expression of MHC antigens on luteal cells was increased by treatment with the T-lymphocyte factor, interferon-gamma (IFN-gamma). Class I and II antigens were elevated 25% and 370% above controls, respectively, after IFN-gamma exposure. Since the corpus luteum is regulated by luteinizing hormone (LH), luteal cells were treated with either hormone alone or hormone in addition to IFN-gamma, and antigen expression was determined. LH treatment attenuated IFN-gamma-induction of Class II antigens on bovine luteal cells. These observations are the first to demonstrate the presence of MHC antigens on bovine luteal cells and the modulation of antigen expression by the lymphokine IFN-gamma and by LH.