Transforming Growth Factor Beta 1 Distribution and Content in the Root Dentin of Young Mature and Immature Human Premolars
Transforming Growth Factor Beta 1 Distribution and Content in the Root Dentin of Young Mature and Immature Human Premolars
复制标题
转化生长因子β1在年轻成熟和未成熟人类前磨牙根牙本质中的分布和含量
DOI：
10.1016/j.joen.2020.01.016
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发表时间：
2020-05-01
影响因子：
4.2
通讯作者：
Zehnder, Matthias
中科院分区：
文献类型：
--
作者：
Ivica, Anja;Deari, Shengjile;Zehnder, Matthias
转化生长因子β 1 （tgf - β 1）是再生牙髓学的关键形态因子；然而，它在牙本质硬组织阶段的位置和在成熟根中的有效性尚未完全阐明。方法：选取13例年龄分别为17 +/- 1岁和12 +/- 1岁的正畸患者的正常前磨牙的幼成熟根（n = 58）和未成熟根（n = 11）。用5%的次氯酸钠清洗根部的有机残留物。用数码显微镜测量小孔的宽度。采用免疫染色联合共聚焦激光扫描显微镜观察每组3根中tgf - β 1的分布。其余13根根的牙本质在17% EDTA中进行粉末化和脱钙，通过酶联免疫吸附法测定硬组织包埋tgf - β 1的总体水平。组间数据比较采用Student t检验（alpha = 0.05）。结果：成熟根与未成熟根的小孔大小分别为168 6 49 mm和557 +/- 295 mm （P < 0.05）。tgf - β 1在两组牙髓间隙高度染色。它明显与小管周围牙本质有关，而在非管状外牙本质中明显不存在。成熟和未成熟根本质中tgf - β 1含量分别为115 +/- 31 pg和74 +/- 35 pg/100 mg （P < 0.05）。结论：tgf - β 1沉积于小管周围牙本质。它应该是可能的释放这种分子再生根管过程中，从年轻的成熟根以及未成熟的根。
Introduction: Transforming growth factor beta 1 (TGF-beta 1) is a key morphogen in regenerative endodontics; yet, its location within the hard tissue phase of dentin and its availability in mature roots have not been fully elucidated. Methods: Young mature (n 5 8) and immature (n = 11) roots from sound premolars were obtained from 13 orthodontic patients aged 17 +/- 1 and 12 +/- 1 years, respectively. Roots were cleaned of organic remnants in 5% sodium hypochlorite. The width of the minor foramen was measured using a digital microscope. TGF-beta 1 distribution was assessed in 3 roots per group by immunostaining combined with confocal laser scanning microscopy. The root dentin of the remaining 13 roots was powdered and decalcified in 17% EDTA to determine the overall levels of hard tissue-embedded TGF-beta 1 by enzyme-linked immunosorbent assay. Data were compared between groups using the Student t test (alpha = .05). Results: The minor foramen was 168 6 49 mm versus 557 +/- 295 mm in mature compared with immature roots (P < .05). TGF-beta 1 was highly stainable toward the pulp space in both groups. It was clearly associated with peritubular dentin and apparently absent in nontubular outer dentin. TGF-beta 1 content was 115 +/- 31 pg and 74 +/- 35 pg/100 mg mature versus immature root dentin, respectively (P > .05). Conclusions: TGF-beta 1 is deposited into the peritubular dentin. It should be possible to release this molecule in regenerative endodontic procedures from young mature roots as well as immature roots.