Comparative expression analysis of transcription factor genes in the endostyle of invertebrate chordates
Comparative expression analysis of transcription factor genes in the endostyle of invertebrate chordates
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DOI：
10.1002/dvdy.20401
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发表时间：
2005-07-01
影响因子：
2.5
通讯作者：
Ogasawara, M
中科院分区：
文献类型：
--
作者：
Hiruta, J;Mazet, F;Ogasawara, M
无脊椎脊索动物的内柱是一个咽部器官，被认为与脊椎动物的滤泡状甲状腺同源。尽管海鞘和文昌鱼内柱的背外侧部分都具有甲状腺样特征，如碘富集和过氧化物酶活性，但其结构组织和功能单位的数量是不同的。为了估计每个功能区的系统发育关系，特别是参考甲状腺的进化，我们已经调查，在海鞘和文昌鱼，甲状腺相关的转录因子，如TTF-2/MoxE 4和Pax 2/5/8，以及叉头转录因子FoxQ 1和FoxA的表达模式。比较基因表达分析描绘了海鞘和文昌鱼endostyles之间的整体相似性，而这些基因的表达模式的差异可能是具体相关的一对腺区的添加或删除。Ci-FoxE和BbFoxE 4的表达表明，祖先FoxE类可能已被招募用于在脊索动物的可能共同祖先中形成甲状腺样区域。此外，共表达FoxE 4，Pax 2/5/8，TPO的背外侧部分海鞘和文昌鱼endostyles表明，甲状腺功能的遗传基础已经到位之前的脊椎动物谱系。(c)2005 Wiley-Liss，Inc.
The endostyle of invertebrate chordates is a pharyngeal organ that is thought to be homologous with the follicular thyroid of vertebrates. Although thyroid-like features such as iodine-concentrating and peroxidase activities are located in the dorsolateral part of both ascidian and amphioxus endostyles, the structural organization and numbers of functional units are different. To estimate phylogenetic relationships of each functional zone with special reference to the evolution of the thyroid, we have investigated, in ascidian and amphioxus, the expression patterns of thyroid-related transcription factors such as TTF-2/MoxE4 and Pax2/5/8, as well as the forkhead transcription factors FoxQ1 and FoxA. Comparative gene expression analyses depicted an overall similarity between ascidians and amphioxus endostyles, while differences in expression patterns of these genes might be specifically related to the addition or elimination of a pair of glandular zones. Expressions of Ci-FoxE and BbFoxE4 suggest that the ancestral FoxE class might have been recruited for the formation of thyroid-like region in a possible common ancestor of chordates. Furthermore, coexpression of FoxE4, Pax2/5/8, and TPO in the dorsolateral part of both ascidian and amphioxus endostyles suggests that genetic basis of the thyroid function was already in place before the vertebrate lineage. (c) 2005 Wiley-Liss, Inc.