Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice
Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice
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DOI：
10.1016/j.rvsc.2012.05.011
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发表时间：
2012-12-01
影响因子：
2.4
通讯作者：
Ozcengiz, Gulay
中科院分区：
文献类型：
--
作者：
Okay, Sezer;Ozcengiz, Erkan;Ozcengiz, Gulay
多杀性巴氏杆菌血清型A：3是一种革兰氏阴性细菌病原体，是引起牛海运热的病原之一。本研究克隆了巴氏杆菌外膜蛋白H（ompH）和脂蛋白E（plpE）基因，构建了plpEC-ompH融合蛋白，并在大肠杆菌中表达。纯化重组PlpE、OmpH和PlpEC-OmpH融合蛋白，并用油基和油基CpG ODN佐剂配制。用两种佐剂配制的重组PlpE和PlpEC-OmpH蛋白接种的小鼠中的抗体应答显著增加（p < 0.05）。然而，仅在用油基CpG制剂免疫后观察到血清IFN-γ水平的显著（p < 0.05）增加。通过用10 LD 50的多杀性巴氏杆菌A：3腹膜内攻击小鼠来评价疫苗的保护性。当与油基CpG ODN一起配制时，重组蛋白PlpE和PlpEC-OmpH融合物赋予100%的保护性，而当在各自的制剂中仅使用油基佐剂时，发现保护性分别为80%和60%。这些结果表明，与油基CpG ODN佐剂配制的重组PIPE或PlpEC-OmpH融合蛋白是可能的针对船舶热的无细胞疫苗候选物。(C)2012爱思唯尔有限公司保留所有权利。
Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil-based and oil-based CpG ODN adjuvants. Antibody responses in mice vaccinated with recombinant PlpE and PlpEC-OmpH proteins formulated with both adjuvants were significantly (p < 0.05) increased. However, a significant (p < 0.05) increment in serum IFN-gamma level was only observed upon immunization with oil-based CpG formulations. Protectivity of the vaccines were evaluated via intraperitoneal challenge of mice with 10 LD50 of P. multocida A:3. The recombinant proteins PlpE and PlpEC-OmpH fusion conferred 100% protection when formulated with oil-based CpG ODN while the protectivity was found to be 80% and 60%, respectively when only oil-based adjuvant was used in respective formulations. These findings indicated that the recombinant PIPE or PlpEC-OmpH fusion proteins formulated with oil-based CpG ODN adjuvant are possible acellular vaccine candidates against shipping fever. (C) 2012 Elsevier Ltd. All rights reserved.