A comparative study of the immunity region of lambdoid phages including shiga-toxin-converting phages: molecular basis for cross immunity.
A comparative study of the immunity region of lambdoid phages including shiga-toxin-converting phages: molecular basis for cross immunity.
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DOI：
10.1266/ggs.75.223
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发表时间：
2000-10-01
影响因子：
1.1
通讯作者：
Sugino, Y
中科院分区：
文献类型：
--
作者：
Fattah, KR;Mizutani, S;Sugino, Y
比较了8个类志贺毒素，包括3个志贺毒素转换酶，已经进行了相对于免疫区域，特别是识别螺旋的阻遏物和CRO蛋白的一方面，和操作序列的另一方面。一些尚未分配的组件的监管电路已推断出计算机搜索。根据它们序列的相似性解释了由λ VT 2-Sa和λ所示的交叉免疫现象。此外，933 W和HK 022在阻遏物和CRO识别螺旋序列以及操纵子识别螺旋序列上的相似性，使我们预测它们将具有相同或相似的免疫特异性。这种同源性也使我们能够定位被认为不存在的噬菌体933 W的O-L（并因此定位P-L）。
Comparison of eight lambdoid phages, including three Shiga-toxin converting phages, has been carried out with respect to the immunity region, especially the recognition helices of their repressor and CRO proteins on the one hand, and operator sequences on the other. Some as yet unassigned components of the regulatory circuits have been inferred by computer search. The cross immunity phenomenon shown by phages VT2-Sa and lambda is explained on the basis of similarity in their sequences. In addition, the similarity of 933W and HK022 in the sequences of their recognition helices of repressor and CRO, on the one hand, and operators, on the other, has led us to predict that they will have identical or similar immunity specificity. This homology has enabled us also to locate the O-L (and consequently P-L) Of phage 933W that has been thought to be non-existent.