Nanoscale cell adhesion ligand presentation regulates nonviral gene delivery and expression
Nanoscale cell adhesion ligand presentation regulates nonviral gene delivery and expression
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DOI：
10.1021/nl062485g
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发表时间：
2007-01-01
期刊：
影响因子：
10.8
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Kong, Hyun Joon;Hsiong, Susan;Mooney, David J.
假设合成ECM中细胞粘附配体的纳米级组织调节非病毒基因递送。这一假设进行了检查与前成骨细胞培养的基板上，目前不同的密度和间距的合成粘附配体。基因转移和表达的水平随着粘附配体的密度而增加，但由于细胞生长速率的变化而随着配体的间距而降低。这项研究为非病毒基因治疗提供了纳米尺度的材料控制点。
It is hypothesized that the nanoscale organization of cell adhesion ligands in a synthetic ECM regulates nonviral gene delivery. This hypothesis was examined with pre-osteoblasts cultured on substrates which present varied density and spacing of synthetic adhesion ligands. The levels of gene transfer and expression were increased with the density of adhesion ligands, but decreased with the spacing of ligands, due to changes in the cell growth rate. This study provides a material-based control point on the nanometer scale for nonviral gene based therapies.