Strain field in actin filament network in lamellipodia of migrating cells: Implication for network reorganization
Strain field in actin filament network in lamellipodia of migrating cells: Implication for network reorganization
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DOI：
10.1016/j.jbiomech.2008.11.012
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发表时间：
2009-02-09
影响因子：
2.4
通讯作者：
Hojo, Masaki
中科院分区：
文献类型：
--
作者：
Adachi, Taiji;Okeyo, Kennedy Omondi;Hojo, Masaki
细胞运动是由参与细胞骨架肌动蛋白结构重组的机械和生物化学因素之间的相互作用在时空上进行调节。虽然细胞运动的分子机制已经得到了很好的研究，机械因素，如应变在网络重组的贡献仍然不清楚。在这项研究中，我们已经定量评估的应变场的肌动蛋白丝网络形成的迁移鱼类角膜细胞的板状伪足，以阐明肌动蛋白丝网络重组的机制是由生物力学因素调节。结果突出了板状伪足中存在负（压缩）应变，其方向平行于细胞运动的方向。结果表明，负应变的分布与肌动蛋白丝的密度密切相关，提示负应变可能参与了肌动蛋白丝的解聚。基于这一结果，我们提出了一个选择性解聚模型，这表明负应变可能与生物力学因素如ADF/cofilin耦合，以促进在变形方向上取向的细丝的选择性解聚，因为这样的细丝经历相对较高的变形水平。这个模型，与其他人一起，可以解释所观察到的丝密度的减少和肌动蛋白丝网络的重组在后面的板状伪足的迁移鱼类角膜基质细胞。就这样。我们认为，通过与生物化学因素耦合，机械因素参与了肌动蛋白丝解聚的调节，从而有助于调节细胞运动。(C)2008爱思唯尔有限公司保留所有权利。
Cell motility is spatiotemporally regulated by interactions among mechanical and biochemical factors involved in the regulation of cytoskeletal actin structure reorganization. Although the molecular mechanisms underlying cell motility have been well investigated, the contributions of mechanical factors such as strain in the network reorganization remain unclear. In this study, we have quantitatively evaluated the strain field in the actin filament network forming the lamellipodia of migrating fish keratocytes to elucidate the mechanism by which actin filament network reorganization is regulated by biomechanical factors. The results highlight the existence of a negative (compressive) strain in the lamellipodia whose direction is parallel to that of cell movement. A close correlation was found between the distributions of the strain and the actin filament density in the lamellipodia, suggesting that negative strain may be involved in filament depolymerization. Based on this result, we propose a selective depolymerization model which suggests that negative strain may couple with biomechanical factors Such as ADF/cofilin to promote selective depolymerization of filaments oriented in the direction of the deformation because such filaments experience relatively higher levels of the deformation. This model, in conjunction with others, may explain the observed reduction in filament density and the reorganization of actin filament network at the back of the lamellipodia of migrating fish keratocytes. Thus. we suggest that by coupling with biochemical factors, mechanical factors are involved in the regulation of actin filament depolymerization, thereby contributing to the regulation of cell motility. (C) 2008 Elsevier Ltd. All rights reserved.