Host gene targets for novel influenza therapies elucidated by high-throughput RNA interference screens.
Host gene targets for novel influenza therapies elucidated by high-throughput RNA interference screens.
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高通量RNA干扰筛查阐明的新型流感疗法的宿主基因靶标。
DOI：
10.1096/fj.11-193466
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Tripp RA
中科院分区：
文献类型：
--
作者：
Meliopoulos VA;Andersen LE;Birrer KF;Simpson KJ;Lowenthal JW;Bean AG;Stambas J;Stewart CR;Tompkins SM;van Beusechem VW;Fraser I;Mhlanga M;Barichievy S;Smith Q;Leake D;Karpilow J;Buck A;Jona G;Tripp RA
流感病毒仅编码11种病毒蛋白，但通过利用宿主细胞功能在广泛的鸟类和哺乳动物物种中复制。全基因组RNA干扰（RNAi）已被证明是鉴定参与病毒复制每个步骤的宿主分子的有力工具。对全基因组RNAi筛选结果的Meta分析表明，流感病毒依赖于宿主细胞途径中的功能节点，需要多种分子和细胞蛋白进行复制。由于甲型流感病毒的快速进化持续使疫苗和治疗剂的有效性复杂化，因此进一步了解流感病毒复制所选择的复杂宿主细胞途径可能为抗病毒治疗提供新的靶点和策略。RNAi基因组筛选技术与生物信息学一起可以提供快速鉴定参与流感病毒抗性和易感性的特定宿主因子的能力，从而允许新的疾病干预策略。Meliopoulos，VA，安徒生湖，澳-地E、Birrer，K. F.、Simpson，K. J.，Lowenthal，J.W.，Bean，A. G. D、Stambas，J.，斯图尔特角，澳-地R.，Tompkins，S. M.，货车Beusechem，V.W.，弗雷泽岛，巴西-地Mhlanga，M.，Barichievy，S.，史密斯，Q。泄漏，D.，Karpilow，J.，巴克，A.，乔纳，G.，特里普河A.通过高通量RNA干扰筛选阐明新型流感治疗的宿主基因靶点FASEB J. 26，1372 - 1386（2012）。http://www.fasebj.org
Influenza virus encodes only 11 viral proteins but replicates in a broad range of avian and mammalian species by exploiting host cell functions. Genome‐wide RNA interference (RNAi) has proven to be a powerful tool for identifying the host molecules that participate in each step of virus replication. Meta‐analysis of findings from genome‐wide RNAi screens has shown influenza virus to be dependent on functional nodes in host cell pathways, requiring a wide variety of molecules and cellular proteins for replication. Because rapid evolution of the influenza A viruses persistently complicates the effectiveness of vaccines and therapeutics, a further understanding of the complex host cell pathways coopted by influenza virus for replication may provide new targets and strategies for antiviral therapy. RNAi genome screening technologies together with bioinformatics can provide the ability to rapidly identify specific host factors involved in resistance and susceptibility to influenza virus, allowing for novel disease intervention strategies.—Meliopoulos, V. A., Andersen, L. E., Birrer, K. F., Simpson, K. J., Lowenthal, J. W., Bean, A. G. D., Stambas, J., Stewart, C. R., Tompkins, S. M., van Beusechem, V. W., Fraser, I., Mhlanga, M., Barichievy, S., Smith, Q., Leake, D., Karpilow, J., Buck, A., Jona, G., Tripp, R. A. Host gene targets for novel influenza therapies elucidated by high‐throughput RNA interference screens. FASEB J. 26, 1372‐1386 (2012). http://www.fasebj.org
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影响因子：
7.3
作者：
Friedman AA;Tucker G;Singh R;Yan D;Vinayagam A;Hu Y;Binari R;Hong P;Sun X;Porto M;Pacifico S;Murali T;Finley RL Jr;Asara JM;Berger B;Perrimon N
通讯作者：
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DOI：
10.1007/978-1-61779-276-2_9
发表时间：
2011-01-01
期刊：
NETWORK BIOLOGY: METHODS AND APPLICATIONS
影响因子：
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作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
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