Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells
Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells
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DOI：
10.1128/iai.70.7.3413-3418.2002
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发表时间：
2002-07-01
影响因子：
3.1
通讯作者：
Wyrick, PB
中科院分区：
文献类型：
--
作者：
Davis, CH;Raulston, JE;Wyrick, PB
沙眼衣原体血清型E是导致性传播疾病的主要细菌因子，其生长和存活需要侵入生殖器上皮细胞，但对促进其进入的粘附素-受体相互作用知之甚少。相比之下，关于结合C的硫酸乙酰肝素受体的研究已经发表了很多。沙眼衣原体L2初级体（EB）进入HeLa细胞之前。使用不同的实验方法，其中在4 ℃下附着在EB上的生物素化顶端膜蛋白受体从极化的HEC-1B细胞表面剥离，并用多克隆抗EB抗体免疫沉淀，通过增强化学发光和二维凝胶电泳可重复地检测到类似于55 kDa的蛋白。基质辅助激光解吸电离质谱序列分析显示，55 kDa的蛋白质是蛋白质二硫键异构酶（PDI），雌激素受体复合物的一员，在感染的宿主细胞表面进行巯基-二硫键交换反应。在EB附着期间（1.5至2小时），HEC-1B细胞暴露于三种不同的PDI还原反应抑制剂-（i）硫醇烷基化试剂DTNB（5，5 '-二硫代双[2-硝基苯甲酸]），（ii）杆菌肽，和（iii）抗PDI抗体-导致衣原体感染性降低。自（i）C.沙眼衣原体血清型E对雌激素主导的原代人子宫内膜上皮细胞的附着显著增强，和（ii）EB在宿主细胞中的生产性进入和感染性依赖于宿主细胞表面EB交联外膜蛋白的减少，这些数据为进一步分析Luminal C提供了一些初步证据。沙眼血清型E条目。
Chlamydia trachomatis serovar E, the leading bacterial agent responsible for sexually transmitted diseases, is required to invade genital epithelial cells for its growth and survival, yet little is known about the adhesin-receptor interactions promoting its entry. In contrast, much has been published on the heparan sulfate receptor for binding C. trachomatis L2 elementary bodies (EBs) prior to entry into HeLa cells. Using a different experimental approach in which a biotinylated apical membrane protein receptor(s) attached to EB at 4degreesC was stripped off the surface of polarized HEC-1B cells and immunoprecipitated with polyclonal anti-EB antibodies, an similar to55-kDa protein was reproducibly detected by enhanced chemiluminescence and two-dimensional gel electrophoresis. Matrix-assisted laser desorption ionization mass-spectrometry sequence analysis revealed the 55-kDa protein to be protein disulfide isomerase (PDI), a member of the estrogen receptor complex which carries out thiol-disulfide exchange reactions at infected host cell surfaces. Exposure of HEC-1B cells during EB attachment (1.5 to 2 h) to three different inhibitors of PDI reductive reactions-(i) the thiol-alkylating reagent DTNB (5,5'-dithiobis[2-nitrobenzoic acid]), (ii) bacitracin, and (iii) anti-PDI antibodies-resulted in reduced chlamydial infectivity. Since (i) C. trachomatis serovar E attachment to estrogen-dominant primary human endometrial epithelial cells is dramatically enhanced and (ii) productive entry into and infectivity of EB in host cells is dependent on reduction of EB cross-linked outer membrane proteins at the host cell surface, these data provide some preliminary evidence for an intriguing new potential receptor candidate for further analysis of luminal C. trachomatis serovar E entry.