Fabrication and investigation of electrochromatographic columns with a simplex configuration.
Fabrication and investigation of electrochromatographic columns with a simplex configuration.
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DOI：
10.1016/j.chroma.2014.05.012
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发表时间：
2014-07
期刊：
影响因子：
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通讯作者：
Qing Liu;Lijun Yang;Qiuquan Wang;Bo Zhang
中科院分区：
文献类型：
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作者：
Qing Liu;Lijun Yang;Qiuquan Wang;Bo Zhang
Duplex capillary columns with a packed and an open section are widely used in electrochromatography (CEC). The duplex column configuration leads to non-uniform voltage drop, electrical field distribution and separation performance. It also adds to the complexity in understanding and optimizing electrochromatographic process. In this study, we introduced a simplex column configuration based on single particle fritting technology. The new column configuration has an essentially uniform packed bed through the entire column length, with only 1 mm length left unpacked serving as the optical detection window. The study shows that a simplex column has higher separation efficiency than a duplex column, especially at the high voltage range, due to the consistent distribution of electrical field over the column length. In comparison to the duplex column, the simplex column presented a lower flow rate at the same applied voltage, suggesting that an open section may support a higher speed than a packed section. In practice, the long and short ends of the simplex column could be used as independent CEC columns respectively. This “two-in-one” bi-functional column configuration provided extra flexibilities in selecting and optimizing electrochromatographic conditions.
